FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756538
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG47830.27229698418138404No Hit
GTAATATAGGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGGAGATACA46410.2642129006033459No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44630.2540793310477769No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42220.24035916103152904No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA34570.1968075840089995No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA32640.18582006196279272No Hit
CAGTAATATAGGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGGAGATA27900.15883516325863714No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27690.1576396297717442No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27620.15724111860944653No Hit
ATATTACTGTGCGAGAAGGGGGTATAACTGGAACTCGCCTGACTGCTGGGGCCAGGGAACCCTGGTCACCGTCTC27570.15695646777923392No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27310.15547628346212836No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26790.15251591482791718No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26430.15046642885038639No Hit
CTATATTACTGTGCGAGAAGGGGGTATAACTGGAACTCGCCTGACTGCTGGGGCCAGGGAACCCTGGTCACCGT26290.14966940652579105No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC26180.14904317469932332No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24950.14204076427609308No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG24840.14141453244962535No Hit
GTCTGGGACAGACTTCATTCTCACCATCAGCAGCCTAGAGCCTGAAGACT24800.14118681178545525No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTCTG24250.13805565265311653No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23910.13612002700767076No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG23790.1354368650151605No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCCTCTTAGCCTGGTA23700.13492449352077782No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22670.12906068641839802No Hit
GTAATAAACTGCAAAGTCTTCAGGCTCTAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACCCACTGCCA22120.12592952728605927No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22040.12547408595771908No Hit
GTTTATTACTGTCACCAGCGTCGCAGCTGGCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA21870.12450627313499622No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCCTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT21150.12040730117993462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21050.1198379995195094No Hit
GCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCACCAGCGTCGCAGCTGGCCCATCACCTTCGGCCAAGGGA20940.11921176769304166No Hit
GTCTTCAGGCTCTAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACC20480.11659298005508563No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC20220.11511279573798006No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19930.11346182092274691No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19490.11095689361687593No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19290.10981829029602548No Hit
GTGTTAGTAGCCTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCC18870.10742722332223954No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC18860.107370293156197No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18360.1045237848540709No Hit
GACTTCATTCTCACCATCAGCAGCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCACCAGCGTCGCAGCTGG18340.10440992452198587No Hit
GAGTGTTAGTAGCCTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC18330.10435299435594333No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18190.10355597203134802No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18180.10349904186530551No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC18060.10281587987279524No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17960.10224657821237002No Hit
GCCCTATATTACTGTGCGAGAAGGGGGTATAACTGGAACTCGCCTGACTG17930.10207578771424244No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17770.10116490505756208No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC17700.10076639389526444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)