FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697297
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56630.8121360051742658No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13020.18672100984229104No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11010.1578954161569604No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10220.14656595396222843No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9990.143267502943509No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9760.1399690519247896No Hit
GTATAATGAGTGGCCTCCTGCGTGGACGTTCGGCCAAGGGACCAAGGTGG8910.12777912424691343No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8860.12706206967762662No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8110.11630625113832414No Hit
CGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGG8040.11530237474132257No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8000.1147287310858931No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7890.11315121103346207No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7850.11257756737803261No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC7650.10970934910088527No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7580.10870547270388371No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC7570.10856206179002635No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7480.10727136356531006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7430.10655430899602321No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7200.10325585797730377No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC7200.10325585797730377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.003770714661.2661470
TAAGCGC200.006874030552.6475647
GTAAGCG200.006931503352.53684246
TAGCGTT200.00831939150.16678610
TATACCG200.00831939150.1667866
GTGGTAT5100.040.7106061
GGTATCA17550.040.070821
GTATCAA42550.038.485341
GACAGGT1450.034.6425061
TATCAAC51750.031.4335692
ATCAACG52750.030.636323
CGGGTAT553.8782894E-430.44122917
TCAACGC52950.030.3876844
AACGCAG53550.030.1656516
CAACGCA54050.029.7628425
ACCGCGT450.005379783529.7284648
CGCAGAG55900.028.9573368
ACGCAGA56500.028.7090157
AGAGTAC57100.028.28817211
GCAGAGT57400.028.200619
Produced by FastQC (version 0.11.5)