FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544198
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21030.38644022947530127No Hit
CCTATGTGCTGACTCAACCACCCTCGGTGTCAGTGGCCCCAGGACAGACG19570.3596117589553802No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18360.3373772046203771No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCGGTTACTACTACTATCCCACAC16530.30374973814677747No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCGGTTACTA16010.29419439248214807No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15920.29254058265557753No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15050.276553754332063No Hit
GGATTACCTGTGAGGGAAACAACATTGGATTTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC14730.27067354161536794No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14120.25946438612416806No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13570.24935777051734845No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13290.24421258439024032No Hit
CCCCTGTGCTGGTCGTCTATGCTGATAGCGACCGGCCCTCAGGGATCCCT13200.24255877456366987No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12610.23171713236726338No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGAGGGAAACAACATTGGATTT12540.23043083583548632No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12190.22399935317660113No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11970.21995670693387334No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11650.2140764942171783No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAAAT11590.21297395433279798No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11310.20782876820568982No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10770.197905909246267No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10580.1944145329457293No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10570.1942307762983326No Hit
CTACTATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGGCTTCGAC10560.19404701965093588No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10410.1912906699399851No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10410.1912906699399851No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10360.19037188670300148No Hit
GGATAGTAGTAGTAACCGGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC9680.17787643468002456No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9540.17530384161647047No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9520.17493632832167705No Hit
CAGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAC9470.17401754508469344No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9390.17254749190551968No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9390.17254749190551968No Hit
GTAGTAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG9360.17199622196332953No Hit
GGGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCAGCATAGA9240.1697911421945689No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8940.16427844277266732No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG8190.15049669421791334No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAACCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC8180.1503129375705166No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8160.14994542427572316No Hit
CTTTTAAATCCAATGTTGTTTCCCTCACAGGTAATCCTGGCCGTCTGTCC8140.14957791098092973No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAACCAC8090.14865912774394613No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7890.14498399479601173No Hit
ATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGGCTTCGACCCTGC7870.1446164815012183No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG7850.14424896820642488No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA7750.14241140173245767No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT7700.14149261849547407No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7680.14112510520068064No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7670.1409413485532839No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCGGTTACTAC7530.13836875548972985No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAACCACCCTCGGTGTCA7500.1378174855475397No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7500.1378174855475397No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT7280.13377483930481185No Hit
GTAGTAGTAACCGGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA7180.13193727283084467No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC7010.12881340982510042No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6960.12789462658811682No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6940.1275271132933234No Hit
GTGCTGGTCGTCTATGCTGATAGCGACCGGCCCTCAGGGATCCCTGAGCG6900.1267920867037365No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGCTGATAGCGACCGGCCCTCAGG6810.12513827687716603No Hit
GACTACTACTGTCAGGTGTGGGATAGTAGTAGTAACCGGGTGGTATTCGG6770.12440325028757915No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC6750.12403573699278571No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6700.12311695375580212No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC6680.12274944046100868No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6660.12238192716621524No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6660.12238192716621524No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG6490.11925806416047101No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT6440.11833928092348742No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA6340.1165017144495202No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6290.1155829312125366No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6130.1126428248541891No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGAGGGAAACAACATTGGATTTAAAAGTGTG6100.11209155491199894No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6100.11209155491199894No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6060.11135652832241205No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAACCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG6060.11135652832241205No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT6050.11117277167501534No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAAC5990.11007023179063502No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCGGTTA5900.10841642196406456No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT5860.10768139537447767No Hit
GTGCTGACTCAACCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG5790.10639509884270064No Hit
CTACTGTCAGGTGTGGGATAGTAGTAGTAACCGGGTGGTATTCGGCGGAG5740.10547631560571705No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC5720.10510880231092361No Hit
CTATCAGCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTA5700.10474128901613017No Hit
ATGCTGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACCCTGA5660.10400626242654328No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC5630.10345499248435312No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5530.10161742601038594No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5480.10069864277340232No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC5480.10069864277340232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA150.002342818869.0892641
AGGGGGG1500.068.8196870
CCATTAT400.007429410751.61475870
TGAATAG354.1251228E-450.13620869
TCTCGTA1350.048.352338
TATACAC2150.047.5149153
TCGTATG1750.047.30489740
CGTATGC1850.046.68193441
ATCTCGT1400.046.58222237
CTTATAC2150.045.935311
GGTATCA5050.045.85691
AATCTCG1500.045.70992336
TTATACA2200.044.88722
CCGTCTT1900.043.9840147
GTCGTAT405.0512448E-543.04829439
CGAGACA2300.042.92374423
ATGTCGT405.1518557E-542.9046837
CGTCTTC1800.042.61870248
CTTGTAT405.3989348E-542.565452
CTCGCTA405.4077274E-542.5537123
Produced by FastQC (version 0.11.5)