FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544198
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20020.3678808080882326No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19910.36585948496686865No Hit
CCTATGTGCTGACTCAACCACCCTCGGTGTCAGTGGCCCCAGGACAGACG18170.33388582831983943No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16940.31128376069004293No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCGGTTACTACTACTATCCCACAC16900.3105487341004561No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16220.29805328207747916No Hit
GGATTACCTGTGAGGGAAACAACATTGGATTTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC15000.2756349710950794No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCGGTTACTA13770.2530329034652829No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12860.23631104855218135No Hit
CCCCTGTGCTGGTCGTCTATGCTGATAGCGACCGGCCCTCAGGGATCCCT12790.23502475202040432No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12420.2282257560667257No Hit
GGGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCAGCATAGA12390.22767448612453556No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12260.2252856497083782No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12230.22473437976618804No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11760.21609781733854225No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11520.21168765780102095No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10860.19955971907283745No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10580.1944145329457293No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10460.19220945317696866No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGAGGGAAACAACATTGGATTT10420.19147442658738179No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAAAT10370.1905556433503982No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10350.19018813005560475No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10200.18743178034465396No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9910.18210283757014908No Hit
CTACTATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGGCTTCGAC9640.17714140809043769No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9470.17401754508469344No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9240.1697911421945689No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9030.1659322525992378No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8940.16427844277266732No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8900.16354341618308044No Hit
GGATAGTAGTAGTAACCGGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC8700.159868283235146No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAACCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC8610.15821447340857556No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8560.15729569017159195No Hit
GTAGTAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG8040.14774034450696255No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8000.14700531791737567No Hit
CAGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAC7990.14682156126997895No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT7860.14443272485382158No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7790.14314642832204455No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7740.14222764508506094No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7690.14130886184807737No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7680.14112510520068064No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA7560.13892002543192No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7320.13450986589439873No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG7210.13248854277303482No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7190.13212102947824136No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG7190.13212102947824136No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT7100.1304672196516709No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6950.12771086994072012No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC6910.12697584335113324No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAACCAC6880.1264245734089431No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGCTGATAGCGACCGGCCCTCAGG6870.12624081676154633No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6850.1258733034667529No Hit
GTAGTAGTAACCGGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA6830.12550579017195948No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAACCACCCTCGGTGTCA6830.12550579017195948No Hit
CTTTTAAATCCAATGTTGTTTCCCTCACAGGTAATCCTGGCCGTCTGTCC6790.1247707635823726No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6660.12238192716621524No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAAC6620.12164690057662837No Hit
GTGCTGGTCGTCTATGCTGATAGCGACCGGCCCTCAGGGATCCCTGAGCG6530.11999309075005789No Hit
GACTACTACTGTCAGGTGTGGGATAGTAGTAGTAACCGGGTGGTATTCGG6520.11980933410266116No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6420.11797176762869396No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT6400.11760425433390052No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG6300.11576668785993334No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC6270.11521541791774316No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCGGTTACTAC6210.11411287803336286No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6070.11154028496980879No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA6070.11154028496980879No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6050.11117277167501534No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5950.10933520520104816No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5860.10768139537447767No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC5750.10566007225311376No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCCGGTTA5730.10529255895832032No Hit
CTACTACTGTCAGGTGTGGGATAGTAGTAGTAACCGGGTGGTATTCGGCG5650.10382250577914656No Hit
ATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGGCTTCGACCCTGC5550.10198493930517935No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGAGGGAAACAACATTGGATTTAAAAGTGTG5520.1014336693629892No Hit
GTGTGGGATAGTAGTAGTAACCGGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG5480.10069864277340232No Hit
GCTTGGTCCCTCCGCCGAATACCACCCGGTTACTACTACTATCCCACACCTGACAGTAGTAGTCGGCCTCATCCC5450.10014737283121217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGA200.0070994852.21821247
GCGATAG200.00713124252.159346
TGGTATC2100.042.1215482
GTATCAA13400.040.1441461
TAACCGT350.0014495138.8456957
GGTATCA5100.037.384171
TATCAAC14950.035.9555662
GTTTTCA400.002339613435.23663754
CTGATTG400.002769162934.04631
TATAAGA400.002779175834.0212482
TAGGGTG400.00279173233.9899835
ATCAACG16300.032.959463
AATGGTA1958.092684E-932.9435370
TCAACGC16500.032.547984
CAACGCA16900.031.7776185
AACGCAG17400.031.255166
CGCAGAG17800.030.361848
AACTAGA450.004911900530.28567927
GTACCAA450.0049296330.2633761
CGGACTT1702.2270942E-629.39079970
Produced by FastQC (version 0.11.5)