FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068262
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31620.29599480277310247No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28160.2636057446581457No Hit
GGGTAACAGGTTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT27740.25967412488696595No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25230.23617801625443946No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT22640.2119330276654978No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22590.21146497769273828No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22400.2096863877962522No Hit
GTAATATAGTGCCGTGTCCTCGGCTCTCAGGCTGGTCAATTGCAGATACA21710.20322729817217125No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21130.19779791848816114No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20640.19321102875511814No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20570.19255575879325484No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19710.18450529926179157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18720.17523790980115364No Hit
CTATATTACTGTGCGACATATAAAAACAGGGCCTCTGACAACTGGGGCCA17980.16831077020431318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17610.16484720040589293No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17070.15979226070009042No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16880.15801367080360437No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTAACAGGTTC16710.15642230089622208No Hit
ATGCTGCATCACGCTTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA15790.14781018139744745No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15340.14359773164261203No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15060.14097665179515886No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15020.14060221181695126No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14600.13667059204577153No Hit
ACCCAGGGTATTGGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGACTCCTGATCTATG14580.13648337205666775No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14530.13601532208390826No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14060.13161565233996902No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14020.13124121236176145No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13690.12815208254154878No Hit
GTAGGAGACAGAGTCACCGTCACTTGTCGGGCGACCCAGGGTATTGGCAACTGGTTAGCCTGGTATCAGCAGAAA13640.12768403256878932No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13230.12384602279216148No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13200.12356519280850578No Hit
ATATTACTGTGCGACATATAAAAACAGGGCCTCTGACAACTGGGGCCAGG13030.1219738229011235No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12620.11813581312449567No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12580.11776137314628808No Hit
GATCTATGCTGCATCACGCTTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA12480.1168252732007691No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12410.1161700032389058No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12400.1160763932443539No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12130.11354892339145266No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGAAC12020.11251921345138177No Hit
CTCCTGATCTATGCTGCATCACGCTTGCAAAGTGGGGTCCCATCGAGGTT11770.11017896358758432No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11690.10943008363116914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.10765149373468305No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11380.10652817380006029No Hit
CTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC11350.10624734381640458No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11340.1061537338218527No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11320.1059665138327489No Hit
GGGTATTGGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCC11240.1052176338763337No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11120.10409431394171093No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11000.10297099400708816No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10930.10231572404522486No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10770.10081796413239448No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10750.10063074414329068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3650.047.3475381
TCGTATG5200.040.4766140
ATGCCGT5450.039.52430344
GTATCAA41450.038.8319471
CGTATGC5600.038.270941
GTATGCC5800.037.60826542
TATACAC5950.037.5878143
GCCGTCT5400.037.44791446
ACGAGAC6000.037.29203822
CCCACGA6000.036.71497319
CGAGACG6100.036.6841323
ATCTCGT4800.036.4595837
TTATACA5950.036.4487882
ATCTCCG6050.035.8396210
CTTATAC6150.035.8144531
GAGCTAC6300.035.6749630
AGACGGA6200.035.57892625
TCTCCGA6100.035.5458511
CACGAGA6350.035.2316221
GGAGCTA6200.035.1237829
Produced by FastQC (version 0.11.5)