FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068262
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51980.4865847516807674No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27950.2616399347725558No Hit
GGGTAACAGGTTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26740.2503131254317761No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25870.24216905590576093No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24870.2328080564505711No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20690.19367907872787762No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19380.18141616944157893No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19210.17982479953419667No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18000.168497990193417No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16830.15754562083084486No Hit
GTAATATAGTGCCGTGTCCTCGGCTCTCAGGCTGGTCAATTGCAGATACA16610.1554862009507031No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16530.1547373209942879No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16460.15408205103242462No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16410.15361400105966513No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15870.14855906135386263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15720.14715491143558415No Hit
CTATATTACTGTGCGACATATAAAAACAGGGCCTCTGACAACTGGGGCCA15690.14687408145192846No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTAACAGGTTC15190.14219358172433355No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14800.13854279193680952No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14030.13133482235631336No Hit
ATGCTGCATCACGCTTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA13920.13030511241624246No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13810.12927540247617159No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13640.12768403256878932No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13340.12487573273223236No Hit
ATATTACTGTGCGACATATAAAAACAGGGCCTCTGACAACTGGGGCCAGG13170.12328436282485009No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12970.12141216293381211No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12830.12010162301008553No Hit
ACCCAGGGTATTGGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGACTCCTGATCTATG12780.11963357303732605No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11950.11186394348951847No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11910.11148950351131089No Hit
CTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC11850.11092784354399951No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11810.11055340356579191No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11480.10746427374557926No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11310.10587290383819699No Hit
GATCTATGCTGCATCACGCTTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11290.1056856838490932No Hit
CTCCTGATCTATGCTGCATCACGCTTGCAAAGTGGGGTCCCATCGAGGTT11140.10428153393081473No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11050.10343904397984763No Hit
GTAGGAGACAGAGTCACCGTCACTTGTCGGGCGACCCAGGGTATTGGCAACTGGTTAGCCTGGTATCAGCAGAAA10880.10184767407246537No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10720.100349914159635No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGAAC10700.10016269417053121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42350.038.5951231
GTGGTAT3950.038.5526431
TATCAAC51000.031.8365962
CGGCTCT7150.031.2316721
CGTTGTC7050.030.84524363
ATCAACG52850.030.582693
CAATAGA1001.7105776E-830.5267126
GCTTGTA1001.7493221E-830.4565871
TCAACGC53300.030.308874
GGCGTTG7150.030.21812261
GACAGGT2800.030.2148721
AACGCAG53400.030.1859956
CAACGCA53800.030.024385
GCGTTGT7200.029.5893262
TTGGCGT7150.029.52156459
CAATTGC7100.029.4989237
GTTGTCT7450.029.32309764
AGTTGTT7250.029.29854254
GAGTTGT7600.028.82577353
ATAGTGC7400.028.7698236
Produced by FastQC (version 0.11.5)