FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108639
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT5130.47220611382652633No Hit
CCTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA2300.21171034343099626No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA1850.17028875449884479No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG1780.16384539622051014No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC1770.16292491646646232No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC1670.15372011892598422No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1630.150038199909793No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC1620.14911772015574518No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT1590.14635628089360175No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1590.14635628089360175No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC1550.1426743618774105No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG1550.1426743618774105No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1430.1316286048288368No Hit
GGCCAAAGGTGTACCCCGGAGGCCAGCTGCTACGTTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGG1410.1297876453207412No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT1380.12702620605859774No Hit
GGGTACACCTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG1350.12426476679645432No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG1340.1233442870424065No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC1290.11874188827216746No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT1270.11690092876407182No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT1260.11598044901002402No Hit
GTTTATTACTGTCAACAACGTAGCAGCTGGCCTCCGGGGTACACCTTTGGCCAGGGGACCAGGCTGGAGATCAAA1260.11598044901002402No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG1260.11598044901002402No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1210.11137805023978499No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG1200.11045757048573716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1200.11045757048573716No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG1190.10953709073168935No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT1170.10769613122359374No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCAGCCATAAGGGCCACTGGCGTCCCAGCCAGGTT1160.10677565146954592No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA1150.10585517171549813No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT1120.1030937324533547No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG1110.10217325269930688No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTTAT1110.10217325269930688No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAACGTAGCAGCTGG1100.10125277294525906No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC1100.10125277294525906No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT1090.10033229319121126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG300.001367286179.0128670
AAATGGG257.870294E-570.0247868
GACAGGA150.00296953465.043741
AATGGGG352.2830201E-456.45354569
CCCGCCG200.00639005353.59446348
AGAAATA200.00839237950.02655833
AATCTCG1200.039.29920236
CTCTTAT505.717069E-639.026241
CTTGAAA1650.038.62952857
TGCTTGA1450.038.41917855
ATCTCGT1150.038.2611837
TGAAAAA1800.038.22375559
CTGCTTG1550.038.17598354
GCTTGAA1600.037.27572656
ATCAACG802.8721843E-936.5871053
CATTCCG451.3345791E-436.4712428
ACATTCC451.3566439E-436.36983527
CGAGACA2150.036.35369523
AACACAT451.3978792E-436.18538724
ATGCCGT1650.036.1719844
Produced by FastQC (version 0.11.5)