FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108639
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17031.56757702114342No Hit
CCTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA2120.19514170785813567No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC1920.17673211277717948No Hit
GGCCAAAGGTGTACCCCGGAGGCCAGCTGCTACGTTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGG1650.1518791594178886No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC1610.14819724040169735No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA1560.1435948416314583No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1460.13439004409098024No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT1370.12610572630454994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC1360.12518524655050212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG1320.1215033275343109No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG1300.11966236802621526No Hit
GGTGTACCCCGGAGGCCAGCTGCTACGTTGTTGACAGTAATAAACTGCAA1300.11966236802621526No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1290.11874188827216746No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT1270.11690092876407182No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1260.11598044901002402No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT1260.11598044901002402No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT1250.11505996925597621No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1230.1132190097478806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT1220.11229852999383279No Hit
GTTTATTACTGTCAACAACGTAGCAGCTGGCCTCCGGGGTACACCTTTGGCCAGGGGACCAGGCTGGAGATCAAA1220.11229852999383279No Hit
GGGTACACCTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG1200.11045757048573716No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA1200.11045757048573716No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG1180.10861661097764155No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTTAT1180.10861661097764155No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC1160.10677565146954592No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1150.10585517171549813No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA1130.10401421220740249No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT1130.10401421220740249No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT1120.1030937324533547No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA1110.10217325269930688No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG1110.10217325269930688No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG1110.10217325269930688No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT1100.10125277294525906No Hit
GCCCCAAGGATGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCAT1090.10033229319121126No Hit
GCCATAAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGG1090.10033229319121126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGA100.004985764162.9468870
GGTATCA603.6379788E-1254.240471
GACAGGA308.3795E-443.3923761
GTATCAA1450.035.910931
GAGCCAT1252.5601735E-432.58937570
CAACGCA1700.030.57355
TCAACGC1700.030.57354
ATCAACG1750.029.7410533
GTGGCCG1100.00541492429.62670570
TACGGGA554.5949974E-429.54482715
AACGCAG1650.029.5312236
AGTGGGG450.006166144328.88827515
AGAGTAC1800.027.0702911
CGCAGAG1800.027.070298
GCAGAGT1800.027.070299
TACGGGG759.179485E-525.99944515
AGTACGG2000.025.98747613
TCTCGCG950.00865736125.72845570
GAGTACG1900.025.64553612
GTGGTGG1600.001085664325.46045170
Produced by FastQC (version 0.11.5)