FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539106
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTC67020.4354475910041284No Hit
CACCTGCACCTTGCGCAGTGGCATAACTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC62750.4077042127052978No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC58340.37905121544584974No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53770.3493586536599818No Hit
GACTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAG48480.31498805150522446No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG46530.30231835883948216No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44330.2880243466012088No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAG42080.27340547044842917No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA40750.26476409032256387No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG39600.25729222028892096No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA39390.25592779184799486No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAGTCTCAC38500.25014521416978425No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT38010.24696154780762342No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT37440.24325809918225258No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT37010.2404642695174991No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAAGAGATGCAGAGAGGGAAGACGG36050.23422688235897982No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35900.23325229061546118No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGAATTTTACTCATCTCTGGG35690.23188786217453508No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGC35320.2294838692071891No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCA35310.22941889642428787No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35220.22883414137817668No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC35220.22883414137817668No Hit
GTAATACACAGCCGTGTCGTCGACTCTCAGGCTGTTCAATTGTAGATACA34940.2270149034569419No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34340.2231165364828673No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC33300.21635936706113806No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32750.21278586400156974No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32130.20875755146169273No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC31850.2069383135404579No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGC31630.20550891231663057No Hit
CCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCA31040.20167551812545725No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30870.2005709808161361No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC30580.1986867701120001No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30380.19738731445397523No Hit
CTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCC30350.19719239610527148No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAG30260.1966076410591603No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG30060.19530818540113545No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG29450.19134484564415966No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29350.19069511781514722No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29020.18855101597940624No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATCTGG28880.18764139701878882No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC28850.1874464786700851No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA28050.18224865603798568No Hit
GACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAG27130.17627116001107138No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT26820.17425700374113284No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA26700.17347733034631793No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA26190.17016371841835456No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAG26100.16957896337224337No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25770.16743486153650236No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25410.16509584135205763No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24940.16204212055569922No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24540.15944320923964952No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTAGG23940.15554484226557494No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC23820.15476516887076006No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23720.15411544104174763No Hit
GTATATCCTGTAGGTAGCAATAGTTATGCCACTGCGCAAGGTGCAGGTGA23360.1517764208573029No Hit
CTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC23360.1517764208573029No Hit
GTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAG23120.15021707406767307No Hit
ATCCTGTAGGTAGCAATAGTTATGCCACTGCGCAAGGTGCAGGTGAGACT22930.14898259119254945No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22540.146448652659401No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAGT22030.1431350407314376No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATCTGGCACAGCAACTCTT21870.14209547620501772No Hit
GGCATAACTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCAGG21620.14047115663248663No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACAC21400.13904175540865932No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC21390.13897678262575808No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTA21340.13865191871125185No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20960.13618295296100463No Hit
GTCTCACCTGCACCTTGCGCAGTGGCATAACTATTGCTACCTACAGGATA20870.13559819791489344No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20850.135468252349091No Hit
GTGTATTACTGTGCGAGAGAGGCAGTGACTGGCCCAGATTACTGGGGCCA20820.13527333400038724No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20390.1324795043356338No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG19810.12871108292736175No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC19630.12754157283513937No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19600.12734665448643565No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19400.12604719882841078No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG19050.12377315142686729No Hit
CTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAAT18990.12338331472945983No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG18660.12123921289371882No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT18640.12110926732791633No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT18590.12078440341341011No Hit
GTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGC18530.12039456671600268No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18490.1201346755843977No Hit
GCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG18380.11941997497248404No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT18180.11812051931445917No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18110.11766570983415046No Hit
ATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18060.11734084591964425No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17980.11682106365643431No Hit
TCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGCCCTGCTGCTTATC17910.11636625417612563No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17890.11623630861032314No Hit
ATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAA17830.11584647191291567No Hit
GCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGC17800.11565155356421195No Hit
GTGGCATAACTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCA17740.11526171686680449No Hit
ACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCC17420.11318258781396473No Hit
AGTATATCCTGTAGGTAGCAATAGTTATGCCACTGCGCAAGGTGCAGGTGAGACTGGCTGATGCTCCAAGAGATG17330.11259783276785355No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC17210.11181815937303863No Hit
CTCTTGGAGCATCAGCCAGTCTCACCTGCACCTTGCGCAGTGGCATAACT17130.11129837710982869No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17060.11084356762952No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17020.110583676497915No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16780.10902432970828521No Hit
GGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTT16750.10882941135958146No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16300.10590563612902557No Hit
CTGCATCTCTTGGAGCATCAGCCAGTCTCACCTGCACCTTGCGCAGTGGC16210.10532088108291437No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16190.10519093551711188No Hit
GTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCT16110.10467115325390194No Hit
GAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTC16040.10421634377359323No Hit
GGATGAGGCTGACTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGT15990.10389147985908702No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTG15690.10194229637204975No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCA15550.10103267741143236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG252.6117382E-455.00922836
TATCGTA200.00742434651.63529238
TAGTGGT553.6714543E-451.33923370
TGTCCGT200.00760759151.3178428
GGTATCA13750.046.2462651
AGGGGGG3450.045.0148470
CGTATGC4450.044.22768441
TCGTATG4350.043.6067840
AGACCGG4950.043.504225
TGGTATC3700.043.4202162
TATACAC5550.043.112273
CTAGCTC405.1247964E-542.94928435
TCTCCGA5550.042.47843611
ATGCCGT4850.042.13947344
GACCGGA5050.041.97677226
TCTCGTA3950.041.8311238
TCTTATA1800.041.7778432
CACGAGA5400.041.76562521
CCGGAGC5250.041.7059328
CTCTTAT1900.041.3847771
Produced by FastQC (version 0.11.5)