FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539106
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTC60630.39392998273023433No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCA57040.3706047536686882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56970.3701499441883795No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56530.3672911417407248No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC52320.33993760013930163No Hit
GACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAG50090.32544866955232454No Hit
CACCTGCACCTTGCGCAGTGGCATAACTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC49830.32375937719689224No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGC39560.257032329157316No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39540.2569023835915135No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAG39390.25592779184799486No Hit
GACTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAG39220.25482325453867377No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA37880.24611690162990724No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG37510.24371290866256126No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG36770.2389049227278693No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT36340.23611109306311584No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG36300.2358512019315109No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACAC35990.23383704566157237No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC35660.23169294382583133No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35650.2316279710429301No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAGTCTCAC35640.2315629982600289No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT33310.21642433984403933No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33200.21570963923212566No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC33020.21454012913990328No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAAGAGATGCAGAGAGGGAAGACGG32610.21187624504095237No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT32600.21181127225805113No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31900.20726317745496411No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30840.2003760624674324No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGAATTTTACTCATCTCTGGG30700.199466443506815No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG30380.19738731445397523No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30290.19680255940786404No Hit
GTAATACACAGCCGTGTCGTCGACTCTCAGGCTGTTCAATTGTAGATACA30010.19498332148662925No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC29770.19342397469699943No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29550.1919945734731721No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29370.19082506338094973No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28980.18829112484780125No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28570.1856272407488503No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGC27740.18023449976804717No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA27510.1787401257613186No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27330.1775706156690962No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC25960.16866934441162598No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25710.16704502483909492No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA25450.1653557324836626No Hit
CTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCC25260.16412124960853897No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25090.16301671229921785No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24800.16113250159508183No Hit
CCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCA24140.1568442979235998No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT24100.15658440679199484No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAG23990.15586970618008117No Hit
CCTTGGTCCCGCCGCCGAACACCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCAGA23960.15567478783137745No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23160.15047696519927803No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAG23130.15028204685057428No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATCTGG22710.1475531899687221No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22270.14469438752106745No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22240.1444994691723637No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22170.14404465969205502No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCAAGAGTTG21980.1428101768169314No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTAGG21870.14209547620501772No Hit
GTATATCCTGTAGGTAGCAATAGTTATGCCACTGCGCAAGGTGCAGGTGA21620.14047115663248663No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATCTGGCACAGCAACTCTT21230.1379372180993382No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21180.13761235418483198No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20410.1326094499014363No Hit
GTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAG20080.1304653480656953No Hit
ATCCTGTAGGTAGCAATAGTTATGCCACTGCGCAAGGTGCAGGTGAGACT20000.12994556580248534No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19820.12877605571026296No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG19790.12858113736155924No Hit
GGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT19430.1262421171771145No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAGT19400.12604719882841078No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA19160.12448785203878095No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18890.1227335869004474No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18790.12208385907143497No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT18610.1209143489792126No Hit
GGCATAACTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCAGG18570.12065445784760764No Hit
GTGTATTACTGTGCGAGAGAGGCAGTGACTGGCCCAGATTACTGGGGCCA18370.11935500218958278No Hit
GTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGC18200.11825046488026167No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT18180.11812051931445917No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18090.117535764268348No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18080.11747079148544674No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18010.11701598200513806No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTA17930.1164961997419281No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17920.11643122695902686No Hit
CTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC17920.11643122695902686No Hit
ACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAGC17880.11617133582742188No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG17560.11409220677458212No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC17550.11402723399168088No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17420.11318258781396473No Hit
GGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTAGGTAGCA17400.11305264224816224No Hit
GTCTCACCTGCACCTTGCGCAGTGGCATAACTATTGCTACCTACAGGATA17300.11240291441914983No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17150.11142832267563119No Hit
GCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG16980.11032378536631005No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16510.10727006456995164No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC16470.10701017343834668No Hit
TCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGCCCTGCTGCTTATC16220.1053858538658156No Hit
GTGGCATAACTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCA16210.10532088108291437No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16090.10454120768809945No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACC16060.10434628933939573No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC16020.10408639820779077No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC16010.10402142542488951No Hit
GCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGC15980.10382650707618578No Hit
GGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTT15970.10376153429328455No Hit
ATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAA15660.10174737802334602No Hit
ACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCC15610.1014225141088398No Hit
GAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTC15580.10122759576013608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG100.0066873706147.8663870
TACGTAT300.001790886273.9331970
TGGTACG200.00581894754.9160967
CGTCAGT200.006869924352.6587655
GGTATCA12200.045.3347131
GTATCAA34300.041.3075681
TGGTATC3500.039.0055472
GTGGTAT3450.038.594121
TATCAAC37400.037.597592
ATAAACG557.976523E-637.2240943
ATCAACG39850.035.106793
TCAACGC40450.034.4083864
AACGCAG41450.033.9875566
CAACGCA41250.033.821575
CGCAGAG43800.032.164028
CCGTTAA851.3319732E-732.1138616
AGAGTAC44650.031.697311
CAGAGTA45550.031.07605610
GCAGAGT45850.030.8747319
TTTGGAT36600.030.30048670
Produced by FastQC (version 0.11.5)