FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863237
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG46380.5372800285437255No Hit
GATTACTACTGTGCAGCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGG46370.5371641855017799No Hit
ACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32560.3771849445748966No Hit
GCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGTCATCCCATG26770.3101118232883901No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25810.2989908912616118No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23160.268292485146026No Hit
GTCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23040.26690236864267863No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTC19390.2246196583325321No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19320.22380875703891284No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGC19230.22276616966140236No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18710.21674233148023084No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18390.2130353541379714No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18270.21164523763462412No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC18150.21025512113127684No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17750.20562139945345254No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA17620.2041154399081596No Hit
GTAATACACACCCGTGTCCTCGGCTCTCAGGCTCTTCATCTGCAGAAACA17010.1970490143494776No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAACCA16960.19646979913974957No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16950.19635395609780396No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16850.1951955256783479No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16230.18801325707772024No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16020.1855805531968625No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT15680.18164188977071186No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15360.17793491242845244No Hit
CCCAAACACTCAGGCTGTCATCCCATGCTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGG15300.17723985417677882No Hit
CTACTGTGCAGCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAG14800.17144770207949844No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14660.16982589949225993No Hit
GTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCC14590.16901499819864071No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14560.1686674690728039No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG14010.1622961017657955No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13710.1588208105074273No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13260.15360787361987496No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13260.15360787361987496No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13210.15302865841014693No Hit
GCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCCAAGG13120.15198607103263645No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12950.15001673931956114No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATACATAATTAGTT12820.14851077977426824No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12730.1474681923967578No Hit
GATTATTCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGA12420.14387705809644397No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12420.14387705809644397No Hit
CCCCTGAGGGCCTCTGATTATTCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATA12410.14376121505449835No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12140.14063345292196697No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12140.14063345292196697No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC11800.13669478949581632No Hit
GCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG11640.13484130082468662No Hit
TCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11380.13182938173410083No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11270.13055510827269914No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11070.128238247433787No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11050.1280065613498958No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC10810.12522632834320122No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGG10770.1247629561754188No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10550.12221440925261544No Hit
GCCCCCAAACTGCTCATGTATAGGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT10300.11931833320397527No Hit
GGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10260.11885496103619285No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10170.11781237365868237No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTACTACTGTGCAG10010.11595888498755268No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10000.11584304194560706No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9950.11526382673587901No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9870.11433708240031416No Hit
CCCAGGAACGGCCCCCAAACTGCTCATGTATAGGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGG9870.11433708240031416No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9700.11236775068723885No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT9670.11202022156140201No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9660.11190437851945642No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9650.1117885354775108No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9570.11086179114194596No Hit
GTGTATTACTGTGCGAGAGATTGGTCCTCACCTATAGCAGTGGCGAATGG9510.11016673289027232No Hit
CAGTAATACACACCCGTGTCCTCGGCTCTCAGGCTCTTCATCTGCAGAAA9490.10993504680638111No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9440.10935583159665306No Hit
TTCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCA9370.1085449303030338No Hit
CCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGTCATCCCATGCTGCACAGTAGTAATCAGCCTCATCCT9260.10727065684163213No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG9250.10715481379968653No Hit
GTGCAGCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAGGGACC9120.10564885425439363No Hit
GAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTC8930.10344783645742711No Hit
ATCCCATGCTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCAC8920.1033319934154815No Hit
GTATAGGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC8920.1033319934154815No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT8880.10286862124769906No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8800.10194187691213422No Hit
ACCACATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTTTCTG8780.10171019082824301No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8770.10159434778629739No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8670.10043591736684132No Hit
GTACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGG8640.1000883882410045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2950.062.5101870
CCGAAAA200.007406376351.66493240
TCTCGTA2300.050.66098838
ATCTCGT2400.048.44157437
GTGGTAT2850.046.0976031
CGTATTC306.273176E-446.05538641
TCGTATT306.3618604E-445.92438540
GCGATCT2500.044.87478634
TCGTATG3200.044.13046340
ACATAGT658.16896E-443.64616470
CGTATGC3350.043.30581341
GCCGTCT2750.043.17545346
GCCAGAG19200.042.4811370
TATACAC4800.041.4017833
GATCTCG2900.041.17421336
CTTATAC4850.040.979711
ATGCCGT3250.040.62119344
TTATACA4900.040.5568472
ACGAGAC4900.040.54744722
CGATCTC2800.040.13955335
Produced by FastQC (version 0.11.5)