FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863237
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47440.5495593909899599No Hit
GATTACTACTGTGCAGCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGG42390.4910586548074283No Hit
ACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33580.38900093485334847No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25440.2947046987096244No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC22710.26307954825847363No Hit
GTCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22410.2596042570001054No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20070.23249698518483336No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC20030.23203361301705094No Hit
GCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGTCATCCCATG19840.22983259522008442No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAACCA19450.2253147165842057No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT17880.2071273589987454No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17430.2019144221111931No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17370.20121936385951947No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17010.1970490143494776No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16750.19403709525889182No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16610.19241529267165333No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16120.18673898361631858No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15550.180135930225419No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15510.17967255805763654No Hit
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG15480.17932502893179972No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTC15300.17723985417677882No Hit
GTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCC15210.17619726679926834No Hit
GTAATACACACCCGTGTCCTCGGCTCTCAGGCTCTTCATCTGCAGAAACA14740.17075264382782482No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGC14520.16820409690502144No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14300.1656555499822181No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG14250.16507633477249006No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA14170.1641495904369252No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14100.16333868914330593No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATACATAATTAGTT13910.1611376713463394No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13660.15824159529769924No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13640.15800990921380803No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC13510.15650394966851514No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC12970.15024842540345235No Hit
CTACTGTGCAGCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAG12880.1492058380259419No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12750.147699878480649No Hit
CCCCTGAGGGCCTCTGATTATTCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATA12350.14306615680282472No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12220.14156019725753183No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11820.13692647557970752No Hit
GGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11480.1329878121535569No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11460.1327561260696657No Hit
GCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCCAAGG11440.13252443998577448No Hit
GCCCCCAAACTGCTCATGTATAGGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT11340.13136600956631841No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11250.13032342218880794No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11190.1296283639371343No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11050.1280065613498958No Hit
GATTATTCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGA11050.1280065613498958No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10970.12707981701433094No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10680.12372036879790833No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT10640.1232569966301259No Hit
TCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10520.12186688012677863No Hit
GTATAGGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC10370.12012923449759452No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10270.11897080407813844No Hit
CTCTTGTTCTGGAACCACCTCCAACATCGGAACTAATTATGTATACTGGTATCAGCACCTCCCAGGAACGGCCCC10260.11885496103619285No Hit
CCCAGGAACGGCCCCCAAACTGCTCATGTATAGGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGG10260.11885496103619285No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10170.11781237365868237No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10140.11746484453284557No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTACTACTGTGCAG9920.11491629761004221No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9880.11445292544225978No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG9650.1117885354775108No Hit
GTGCAGCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAGGGACC9460.10958751768054427No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9370.1085449303030338No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT9040.10472210991882878No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9020.10449042383493758No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG8970.10391120862520953No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8940.1035636794993727No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8790.1018260338701886No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC8780.10171019082824301No Hit
GTGTATTACTGTGCGAGAGATTGGTCCTCACCTATAGCAGTGGCGAATGG8720.10101513257656937No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC8670.10043591736684132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATTA301.0673652E-555.9976043
AACTATG200.00560568955.43441864
CTTGCTA252.927081E-453.751479
GTGGTAT2700.043.5865251
CTTGATT405.8264734E-542.0225522
GGTCGTA400.002889211533.75503529
GACAGGT1700.033.623891
GCTTGTG502.1646102E-433.623891
GTATCAA24950.033.017451
TATCAAC25850.030.6919082
ACCACGC553.8056658E-430.5406118
TCTGGAG6500.030.49224970
ATCAACG26350.030.0920733
TCAACGC26750.029.638664
AACGCAG27250.029.3414026
CAACGCA27350.028.9884535
CGCACTT500.006513064728.58838754
TCTGTTA953.9820952E-728.3378625
GCCAGAG12200.028.2798570
CAATGAA1306.021699E-428.23356670
Produced by FastQC (version 0.11.5)