FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218589
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16050.13170970688230405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6000.057.75034370
GTCGTAT350.001364058839.33272639
TTATACA12100.039.1368642
TATACAC12000.038.9032483
GGTATCA22450.038.5971871
GTATCAA55550.037.6665881
CGAGACT12100.037.20458623
CTTATAC12750.036.087991
CACGAGA12300.036.0459721
ACGAGAC12500.036.0066522
TCGTATG3450.034.0103340
CGAGCCC12800.033.84097715
CGTATGC3600.033.6611741
CCCACGA13350.033.45837419
CGCGTAT1154.9112714E-1132.9197839
ACACATC14100.032.8696026
ATCTCGT3750.032.86944237
GACTCCT13550.032.5489726
TACACAT14550.032.3146555
GTGGTAT6900.032.1252981
Produced by FastQC (version 0.11.5)