FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218589
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96620.7928842292192034No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13710.11250717017796813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57400.037.1812061
GGTATCA23050.033.6823731
TGGTATC9900.033.2535742
GTGGTAT10200.032.950461
ATCAACG67800.031.300873
TCAACGC68900.030.7809224
AACGCAG69050.030.6616826
CAACGCA69250.030.573135
CGCAGAG70950.029.887878
TGTGTCG10700.029.47828970
ACGCAGA72000.029.4054077
AGAGTAC72400.028.9648811
CAGAGTA72850.028.87807810
TATCAAC73650.028.8721032
GCAGAGT73450.028.687869
ACCGTAT606.3812E-427.9607648
ACGTACC855.4990087E-627.6318135
ATATCCG500.00886002426.8423335
CGAGACG1558.0340455E-526.54286270
GAGTACG59500.025.43254712
Produced by FastQC (version 0.11.5)