FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788062
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGTTCCAACGATAGGAACTACTTAGCTTGGTACCAGCAGAA58880.7471493359659519No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41360.5248318025739092No Hit
ATATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT38910.4937428780984237No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33900.43016919988528823No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCGTTGGAACTGTATAAAACACT28530.36202735317779566No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA28530.36202735317779566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28330.35948948179204177No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25390.32218277242145926No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC24870.315584306818499No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22950.2912207415152615No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT22630.2871601472980552No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21370.2711715575678056No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA20320.25784773279259754No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20250.25695947780758366No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19500.24744246011100648No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18960.24059020736947093No Hit
TTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT18890.23970195238445707No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18800.2385599102608678No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18670.23691029386012774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18560.2355144645979631No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA18300.23221523179648304No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA17850.22650502117853671No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17790.22574365976281052No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGTTCCAA17510.22219063982275508No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17440.2213023848377412No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16780.2129274092647533No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16760.2126736221261779No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16360.20759787935467006No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15900.20176077516743607No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT15350.1947816288566128No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC15340.1946547352873251No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14260.180950229804254No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14230.1805695490963909No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14080.17866614555707547No Hit
GTTCCAACGATAGGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGG13810.17524001918630766No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA13710.1739710834934307No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13700.17384418992414302No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13700.17384418992414302No Hit
ATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA13540.1718138928155399No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13530.1716869992462522No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGC13510.1714332121076768No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13310.16889534072192292No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13150.16686504361331977No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13070.1658498950590182No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC13050.1655961079204428No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12920.16394649151970278No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGTTCCAACGATAGGAACTA12770.16204308798038733No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12470.15823628090175645No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12170.1544294738231256No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC12020.15252607028381016No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATT11740.1489730503437547No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG11740.1489730503437547No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT11720.14871926320517928No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11370.14427798828010993No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCGTTGGAACTG11350.14402420114153455No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11250.1427552654486576No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTT11130.14123254261720525No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11070.14047118120147908No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG10990.1394560326471775No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10850.13767952267714978No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10840.1375526291078621No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10740.1362836934149851No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10730.13615679984569742No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10410.13209620562849117No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10410.13209620562849117No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10360.13146173778205267No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10340.1312079506434773No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10200.12943144067344955No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTC10190.12930454710416184No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC10130.12854318568843567No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10130.12854318568843567No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9800.12435569790194172No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9740.12359433648621554No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9740.12359433648621554No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9600.12181782651618782No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9550.12118335866974933No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA9420.11953374226900929No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9410.1194068486997216No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9400.1192799551304339No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9200.11674208374467998No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9100.11547314805180303No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9050.11483868020536454No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA9040.11471178663607685No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA9030.11458489306678916No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8970.11382353165106299No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8940.1134428509431999No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8930.1133159573739122No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8870.11255459595818602No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC8750.11103187312673367No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT8520.10811332103311669No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGAGTA8520.10811332103311669No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8400.10659059820166433No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8350.10595613035522586No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8340.10582923678593815No Hit
TTATTACTGTCAGCAATATTATAGTACTCCTCTCACTTTCGGCGGAGGGA8320.10557544964736278No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8310.10544855607807507No Hit
GCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT8300.10532166250878738No Hit
AAGTAGTTCCTATCGTTGGAACTGTATAAAACACTCTGGCTGGACTTGCA8250.1046871946623489No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8160.10354515253875965No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG8150.10341825896947195No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8100.10278379112303347No Hit
GCTAAGTAGTTCCTATCGTTGGAACTGTATAAAACACTCTGGCTGGACTT8000.10151485543015651No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG7980.10126106829158113No Hit
ATTATAGTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA7930.10062660044514264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2000.049.636322
GTGGTAT2150.047.7715641
GGTATCA8150.044.5281831
ATGCCGT2150.038.66888844
AGACTAG3400.037.26423625
GTATCAA20350.037.180441
CTAGCGC3000.036.572128
GTAGTTT1653.1595846E-936.284670
ACGGGTC853.2014214E-936.245595
ATCTCGT2000.036.17774637
TAGCGCT3050.035.99086429
CTTATAC3250.035.8164251
GACTAGC3350.035.7942526
CGAGACT3350.035.773823
ACGAGAC3350.035.7715322
ACTAGCG3100.035.37436727
TCTCCGA3500.035.1988311
CGTGTTG400.002493572434.7838648
CCGTCTT1900.034.74576647
GCCGTCT1900.034.70552446
Produced by FastQC (version 0.11.5)