FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597435
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG47690.2985410986988516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41440.2594158760763349No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35670.22329547055122745No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGACCTCATTTTCAGGGAAA33680.21083799966821812No Hit
GTAGGAGACAGAGTCATCATCACTTGCCAGGCGAGCCAGGACGTTAGCAAGTATTTAAATTGGTATCAGCAGAAA32050.20063414160826576No Hit
CAATAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTAAAAGTAAACTCTGTCCCAGATCCACTTCCA30120.1885522728624326No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29950.18748806680710012No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28930.1811028304751054No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26550.16620394570045102No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAACTGGATCCGGCAGTC26310.16470153715174637No Hit
GAGTTTACTTTTACCATCAGTAGCCTGCAGCCTGAAGATGTTGCAACATATTATTGTCAACAATATGATCATGTC25980.1626357253972775No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24740.1548732812289702No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACGT23920.149740052020896No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23430.1466726345672907No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATACTTGCTA22930.14354261675748936No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC22810.14279141248313704No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22340.13984919574192375No Hit
AAGTAAACTCTGTCCCAGATCCACTTCCACTGAACTTTGGTGGGACCCCT22240.13922319217996348No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22190.13891019039898336No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAAGGAACTGTGGCTGCACCA22100.13834678719321913No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC21660.13559237152059395No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21560.1349663679586337No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21510.13465336617765356No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21210.13277535549177275No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21070.13189895050502837No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT20790.13014614053153964No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20640.12920713518859922No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20410.1277673269960906No Hit
ATCATGTCCCGTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAAGGA20240.12670312094075817No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20100.1258267159540138No Hit
GGCTACTGATGGTAAAAGTAAACTCTGTCCCAGATCCACTTCCACTGAAC19780.12382350455574093No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT19770.1237609041995449No Hit
GTCCCAGATCCACTTCCACTGAACTTTGGTGGGACCCCTGTTTCCAAATT19720.12344790241856475No Hit
GTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCAGGCGAGCCAGG19720.12344790241856475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19230.12038048496495946No Hit
ATCATATTGTTGACAATAATATGTTGCAACATCTTCAGGCTGCAGGCTAC19030.11912847784103893No Hit
AGCCAGGACGTTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAA18690.11700006573037401No Hit
CCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCA18630.11662446359319784No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTT18550.11612366074362963No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18200.11393264827676869No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18140.11355704613959254No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18090.11324404435861239No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17830.11161643509751572No Hit
TCTTCAGGCTGCAGGCTACTGATGGTAAAAGTAAACTCTGTCCCAGATCCACTTCCACTGAACTTTGGTGGGACC17440.10917502120587066No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACGTCCAGATGACCCAGTCTCCATCTTCC17330.10848641728771437No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGA17230.1078604137257541No Hit
GTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGA17070.10685880802661767No Hit
TGATCATATTGTTGACAATAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTAAAAGTAAACTCTGTC16750.1048555966283448No Hit
ATATTATTGTCAACAATATGATCATGTCCCGTACACTTTTGGCCAGGGGA16650.10422959306638455No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16360.10241418273669978No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAATTACTACTGGAACTGGATCCGGCAGTCCCCAGGGAAGGGA16300.10203858059952362No Hit
GGCCAAAAGTGTACGGGACATGATCATATTGTTGACAATAATATGTTGCA16190.10134997668136733No Hit
ATAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTAAAAGTAAACTCTGTCCCAGATCCACTTCCA16140.10103697490038718No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16060.10053617205081898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5650.048.2609021
GTATCAA43100.039.14551
CACTGAT11200.038.04811570
TATCAAC49200.034.203522
GTGCTAG807.8955054E-834.0842631
GACAGGT2650.033.4411661
TCAACGC50900.032.7080964
ATCAACG51150.032.6874923
AACGCAG52050.032.375876
CAACGCA51950.032.1760645
ATACGTA553.5227046E-430.94786511
AATACGT553.523351E-430.94689610
ACGCAGA54850.030.7852027
AGAGTAC56050.030.42884611
CGCAGAG55600.030.3699348
GCAGAGT56600.029.8925769
CAGAGTA57450.029.56788610
GAATACG605.863512E-428.3688779
TGGATTG14900.028.13107570
CGTTTCA650.00850916327.06568769
Produced by FastQC (version 0.11.5)