FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251073
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49500.3956603651425616No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38130.30477837824011866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37770.30190084831180913No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34160.2730456176418163No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32880.2628144001189379No Hit
GTTATTATGCTGCTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT31460.2514641431794947No Hit
GCATAATAACTGGTCATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT30180.24123292565661636No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29600.2365969052165621No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28170.22516671689022144No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27190.21733344097426768No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26590.21253755776041847No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26320.2103794103141863No Hit
CTAGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT26320.2103794103141863No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATTTTGCAGTTTA26220.20958009644521142No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT25460.20350531104100242No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25420.20318558549341248No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24970.19958867308302553No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24820.19838970227956323No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23680.1892775241732497No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22470.17960582635865371No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22420.1792061694241663No Hit
CTCCTGCACGGCCAGTCAGAGTGTTCGCAGCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT22300.17824699278139644No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACTAGTGGCCCTGGT21630.1728915898592648No Hit
GTTTATTACTGTCAGCAGCATAATAACTGGTCATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21580.17249193292477738No Hit
CATCTATGGTGCATCCACCAGGGCCACTAGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA21240.1697742657702628No Hit
GTATCCAGCCACCCTGTCTGTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTGTTCGCAG20730.16569776503849096No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20590.16457872562192613No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20570.16441886284813118No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20210.16154133291982162No Hit
GTCTGTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGA20070.16042229350325682No Hit
GGATACTAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGG19780.15810428328322967No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19200.1534682628431754No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGC19150.15306860590868798No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18630.14891217379001864No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18360.14675402634378648No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18280.1461145752486066No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17270.1380415051719604No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACTA17160.13716225991608802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16870.1348442496960609No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16620.13284596502362372No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA16560.1323663767022388No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15820.12645145407182476No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15270.12205522779246294No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15230.12173550224487299No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15080.12053653144141069No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15010.11997701173312829No Hit
CTAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC14980.11973721757243581No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14920.11925762925105089No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14790.11821852122138356No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14600.1166998248703313No Hit
GTCAGAGTGTTCGCAGCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG14560.11638009932274135No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14250.11390222632891925No Hit
GGAGAAATAGTGATGACGCAGTATCCAGCCACCCTGTCTGTGTCTCCTGG14100.11270325552545696No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14020.11206380443027704No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13750.10990565698404489No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13600.1087066861805826No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13460.10758764676401776No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13300.10630874457365797No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13290.10622881318676049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT259.877358E-485.8869870
TGATTAG400.006365797553.6793670
GTATCAA35550.039.4013941
CGTTTAC400.002268446835.46290260
ATCAACG40850.034.2674033
TATCAAC40850.034.112932
TCAACGC41150.033.7665754
CAACGCA42000.033.0779155
AACGCAG42700.032.854636
ACGCAGA45800.030.6308467
CGCAGAG45700.030.5488558
AGAGTAC46850.030.0122311
GCAGAGT47300.029.4434979
GACAGGT2450.029.2120881
TCGTTAA1255.5545015E-428.62899270
ATAGTTC500.00823629227.2471713
GCTAGTA756.7319736E-527.2449914
CAGAGTA53400.026.20658510
ATTCACT1901.8189894E-1225.1051122
GATTCAC2100.024.3434071
Produced by FastQC (version 0.11.5)