FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211188
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40350.33314398755601937No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38980.3218327790565957No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34950.2885596620838384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28940.23893895910461466No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28790.23770050561927628No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26500.21879344907644394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26230.2165642328028349No Hit
GTCTATGGTGGGTCCTTCAGTGGTTCCTTCTGGAGCTGGATCCGCCAGTC24930.20583096926323577No Hit
GTAATATACAGCCGTGTCCGCGACGCTCACAGAGCTCAGCTTCAGGGAGA23990.1980699940884487No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA23680.19551052355208276No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23650.19526283285501508No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22820.18841005690280946No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21900.18081420885940083No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21630.1785849925857918No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGCCTATTTAAATTGGTATCAGCAAAAA21160.17470450499839826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20450.16884249183446337No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20110.16603533060102973No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20100.1659527670353405No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19380.16000819030571636No Hit
GCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT19160.15819179186055346No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGC18970.15662308411245818No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18530.15299028722213232No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC18450.1523297786966185No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18150.1498528717259418No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17480.1443211128247638No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16580.1368903919127336No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16380.13523912059894913No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCAAAGGGCA16160.1334227221537862No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16090.13284477719396165No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16070.1326796500625832No Hit
CTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTT15660.12929454386932498No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15590.12871659890950043No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15510.12805609038398663No Hit
GGGTTCCAGTGCCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT14840.12252433148280861No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT14780.12202895008867327No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14680.12120331443178102No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGGCACTTACTACTGTCAACAGGGTTCCAGTGCC14630.12079049660333491No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14550.1201299880778211No Hit
GTATATTACTGTGCGAGGAGGGCTAGTGGCTCCGTCCGCTTCCACTTCTA14370.11864384389541507No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14120.11657975475318448No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14000.11558899196491379No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13940.11509361057077845No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13900.11476335630802154No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13720.1132772121256155No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13460.11113055941769569No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13350.11022236019511422No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13320.10997466949804655No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13170.10873621601270819No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13110.10824083461857283No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12930.1067546904361668No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12840.1060116183449638No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12710.10493829199100388No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12650.10444291059686854No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12490.10312189354584095No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGGTGGGACCCCA12400.10237882145463792No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12260.10122293153498878No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12250.10114036796929957No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12220.10089267727223189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATT200.007406364751.66628339
ATATCGT200.00748129651.5349137
GACCGGA1154.438334E-947.2295770
CTAGCGC2550.041.47125228
TGGATTG8700.041.35980270
ACTAGCG2700.039.14137327
GTAGACG350.001424598938.98679728
TAGCGCT2850.037.1212229
GTATCAA40300.036.5741921
GACTAGC3000.035.21412726
CGAGACT3100.035.14986823
CGTATGC2200.034.53800641
GCGTATG400.002596754634.49897440
CTTGCGT400.002762849434.0655125
ACGAGAC3250.033.52202622
CCCACGA3300.033.00321219
TCGTATG2100.032.85616740
CTTATAC3550.032.6018681
CGCTCAT2650.032.27332732
GACAGGT2850.032.2485961
Produced by FastQC (version 0.11.5)