FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880434
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31770.36084476519534686No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28110.3192743578735033No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCACTACCAGTGTCCCACAC25770.29269655647101317No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24880.282587905510237No Hit
CTTATGTCCTGACTCAGCCACCCTCGGTGTCAATGGCCCCGGGACAGACG23770.26998048689623527No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22570.2563508451513685No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCACTA22090.25089898845342185No Hit
GGATTACCTGTGAGGGAAGCAACCTTGAACCTAAAAGTGTGCACTGGTACCAACAGAAGCCCGGCCAGGCCCCT21450.2436298461894929No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20840.23670144496918563No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19150.21750636617849833No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18880.21443969678590333No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18460.20966932217519998No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17690.20092363538891048No Hit
CTTTTAGGTTCAAGGTTGCTTCCCTCACAGGTAATCCTGGCCGTCTGTCCCGGGGCCATTGACACCGAGGGTGG17630.20024215330166714No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17060.19376807347285543No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16930.19229152895049487No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16430.1866125115568004No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16060.18241003868546649No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15800.17945694964074535No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15550.17661744094389814No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15260.1733236108555553No Hit
ACCTAAAAGTGTGCACTGGTACCAACAGAAGCCCGGCCAGGCCCCTCTGC15180.17241496807256423No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14640.1662816292873742No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14560.16537298650438306No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14450.1641236026777703No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14430.1638964419820225No Hit
CTACCAGTGTCCCACACCTGACAGTAAAAGTCGGCCTCATCCCCGACTTC14380.16332854024265306No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14020.15923964771919302No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13630.15481001415211135No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCACCTTCAGGGACA13090.14867667536692133No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTGTGACCTCTTATGTCCTGACT13000.1476544522360563No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTGTGA12940.14697297014881297No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGGTCTGGGAACACGG12500.14197543484236183No Hit
GGTTCTGTGACCTCTTATGTCCTGACTCAGCCACCCTCGGTGTCAATGGCCCCGGGACAGACGGCCAGGATTAC12390.14072605101574906No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12380.14061247066787516No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12150.13800012266677572No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGTCGGGGATGAGGCCGACTTT11800.13402481049118956No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11760.13357048909969402No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11340.12880011448899065No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT11330.12868653414111678No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11140.12652850753151285No Hit
GGGTGGCCGTGTTCCCAGACCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCAAAGA11100.1260741861400173No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10920.1240297398782873No Hit
CCTCTCTCACTGCACAGGTTCTGTGACCTCTTATGTCCTGACTCAGCCAC10890.12368899883466564No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC10850.12323467744317007No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10760.12221245431230507No Hit
GTCCCACACCTGACAGTAAAAGTCGGCCTCATCCCCGACTTCGACCCTGC10690.12141739187718785No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10530.11960010631120561No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG10480.11903220457183615No Hit
GTGTATTACTGTGCGAGAAATGAGAGAGATACTGTGATTCGGGGAATTAT10340.1174420797016017No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10200.11585195483136727No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10170.11551121378774558No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10150.11528405309199781No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10140.11517047274412391No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10100.11471615135262836No Hit
GTGTGGGACACTGGTAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG9940.11289886578664612No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTGTGACCTCTTA9900.11244454439515057No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9870.1121038033515289No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9670.10983219639405112No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9530.10824207152381667No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTGTGACCTCTTATGTCCTGACTCAGC9510.10801491082806887No Hit
CACTGGTAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCC9450.10733342874082553No Hit
GCACAGGTTCTGTGACCTCTTATGTCCTGACTCAGCCACCCTCGGTGTCA9390.1066519466535822No Hit
CAGGTAATCCTGGCCGTCTGTCCCGGGGCCATTGACACCGAGGGTGGCTGAGTCAGGACATAAGAGGTCACAGAA9210.1046075003918522No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9110.1034716969131133No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9100.10335811656523941No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCACTAC9090.10324453621736553No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCA9080.10313095586949163No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9060.10290379517374386No Hit
ATCACTACCAGTGTCCCACACCTGACAGTAAAAGTCGGCCTCATCCCCGA9010.10233589343437441No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTG8960.10176799169500496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG400.00693492752.5270470
TACGGAT4050.051.8785670
GTGGTAT3000.046.457661
TCGTATG1900.045.31778340
ATCTCGT1550.044.2420237
CGTATGC1950.044.23472641
ATGCCGT2000.043.2986544
TCTCGTA1600.042.92341238
TGGTATC3400.041.9847032
GGTATCA10350.040.3979641
CACGAGA2200.040.16262421
TATACAC2550.039.985433
TTATACA2600.039.216482
CCGTCTT2150.038.86017247
GTATGCC2250.038.4054242
CGTCTTC2300.037.89230748
CTTATAC2700.037.7704541
ATCTCCG2450.037.4472310
ACGAGAC2500.036.7045422
GTATCAA25450.036.0637361
Produced by FastQC (version 0.11.5)