FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880434
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28630.32518053596294555No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25780.29281013681888707No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24190.2747508615069386No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCACTACCAGTGTCCCACAC23930.27179777246221754No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22310.2533977561066474No Hit
CTTATGTCCTGACTCAGCCACCCTCGGTGTCAATGGCCCCGGGACAGACG21500.24419774792886237No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCACTA20540.23329403453296896No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20510.2329532934893473No Hit
GGATTACCTGTGAGGGAAGCAACCTTGAACCTAAAAGTGTGCACTGGTACCAACAGAAGCCCGGCCAGGCCCCT19850.22545699052967058No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18690.2122816701762994No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18380.20876067939220883No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18060.20512610826024438No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17970.20410388512937938No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17940.2037631440857577No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17320.19672116251757657No Hit
CTTTTAGGTTCAAGGTTGCTTCCCTCACAGGTAATCCTGGCCGTCTGTCCCGGGGCCATTGACACCGAGGGTGG16320.1853631277301876No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15630.17752608372688924No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15260.1733236108555553No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14980.17014336111508643No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14930.16957545937571697No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14550.1652594061565092No Hit
GGGTGGCCGTGTTCCCAGACCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCAAAGA14090.16003471015431028No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13610.15458285345636358No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13530.15367421067337245No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13450.15276556789038134No Hit
ACCTAAAAGTGTGCACTGGTACCAACAGAAGCCCGGCCAGGCCCCTCTGC13000.1476544522360563No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12930.14685938980093907No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12830.14572358632220017No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTGTGACCTCTTATGTCCTGACT12720.1444742024955874No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12560.14265691692960517No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12360.1403853099721274No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12060.13697789953591072No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12030.13663715849228905No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGGTCTGGGAACACGG11890.13504703362205459No Hit
CTACCAGTGTCCCACACCTGACAGTAAAAGTCGGCCTCATCCCCGACTTC11830.13436555153481125No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCACCTTCAGGGACA11830.13436555153481125No Hit
GGTTCTGTGACCTCTTATGTCCTGACTCAGCCACCCTCGGTGTCAATGGCCCCGGGACAGACGGCCAGGATTAC11690.13277542666457678No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTGTGA11520.13084456075072068No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11380.1292544358804862No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11180.12698282892300844No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11140.12652850753151285No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT11100.1260741861400173No Hit
GTGTGGGACACTGGTAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG10790.12255319535592674No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10580.12016800805057506No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG10580.12016800805057506No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10380.11789640109309728No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10200.11585195483136727No Hit
CCTCTCTCACTGCACAGGTTCTGTGACCTCTTATGTCCTGACTCAGCCAC10010.11369392822176336No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9990.11346676752601557No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGTCGGGGATGAGGCCGACTTT9750.11074083917704224No Hit
GTCCCACACCTGACAGTAAAAGTCGGCCTCATCCCCGACTTCGACCCTGC9710.11028651778554667No Hit
GCACAGGTTCTGTGACCTCTTATGTCCTGACTCAGCCACCCTCGGTGTCA9700.11017293743767279No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9700.11017293743767279No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC9590.10892355361106No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9570.10869639291531223No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTGTGACCTCTTATGTCCTGACTCAGC9550.10846923221956445No Hit
GTTGGTACCAGTGCACACTTTTAGGTTCAAGGTTGCTTCCCTCACAGGTA9460.10744700908869945No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9420.10699268769720387No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9380.10653836630570833No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9110.1034716969131133No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTGTGACCTCTTA8980.10199515239075274No Hit
GTGTATTACTGTGCGAGAAATGAGAGAGATACTGTGATTCGGGGAATTAT8860.10063218821626607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT4000.048.86185570
GTGGTAT4100.041.4902951
TGGTATC4200.038.851392
GGTATCA10850.037.9416281
GTATCAA28300.037.5083961
TGCGGCA500.002168609535.7873969
CTACGGA3850.034.8578569
GTGTTAG502.0200304E-434.022041
CGGGTAT906.009941E-933.98338317
TATCAAC31200.033.4501762
ATCAACG32200.032.292933
TCAACGC32350.032.0326924
AACGCAG33300.031.3211296
CAACGCA33150.031.2578835
TCGGGCT450.004980230730.20230113
CGCAGAG34750.029.9164378
GTAGTTT1703.0425344E-629.80679570
AGAGTAC35400.029.74931711
ACGCAGA35050.029.6603747
GACAGGT1950.029.6602381
Produced by FastQC (version 0.11.5)