FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870516
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23280.26742759466799No Hit
GGGTTACAGTACCCCACTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22770.2615690004549026No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20320.23342477335281603No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17670.2029830583240285No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16350.18781963800780227No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16280.18701551723345694No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15580.17897430949000362No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15210.17472395682560687No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15200.17460908242927184No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14100.16197289883241664No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13470.15473581186330868No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13020.14956646402823154No Hit
CTGTAACCCTGTTGACAGTAGTAAATTGCAAAATCTTCAGGTTGCAGATTGCTGATGGTGAGAGTGAAATCTGTC12640.14520123696749973No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12380.1422145026627885No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12200.14014676352875766No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGGTCAACTTCAGGGACA12130.13934264275441233No Hit
GTCTCAGTCAGGACGCAGCATGGACATGAGGGTCCCCGCTCCGCTCCTGG11430.131301435010959No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10940.1256725895905417No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10690.12280072968216553No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10290.11820575382876362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10290.11820575382876362No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10180.1169421354690781No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10160.11671238667640801No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10140.11648263788373793No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10100.11602314029839775No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9820.11280665720101642No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9510.10924555091462994No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9310.106948062987929No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9000.10338695670154252No Hit
GTCTCCATCCTCCCTGTCTGCATCCGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCAG8910.10235308713452711No Hit
GTCCCTGGTGGCTCCATCAGTACTTACTACTGGAGTTGGATCCGACAGTC8860.10177871515285188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT1750.042.6165961
GTGGTAT2950.039.0706831
TACGTAT700.001411518939.05436370
GTATCAA30750.037.151721
GTTATAG400.002830149133.8976173
ATCAACG36450.031.2471853
TCTTATA2500.031.1858042
AACGCAG36800.030.9499956
CAACGCA37350.030.5855
TCAACGC37050.030.5581744
AGTACTC450.00505209430.1139078
TAACGGC802.9295552E-629.95827536
ACGAGAC3900.029.55176722
CGCAGAG38750.029.2007738
ACGCAGA38800.029.1631457
GACAGGT2450.029.0567681
AGAGTAC39600.028.48843811
GCAGAGT39950.028.3236569
TTGCCGT500.0074849627.78558544
TATCAAC41150.027.6782472
Produced by FastQC (version 0.11.5)