FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144999
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC66740.5828826051376464No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCG44170.38576452905199043No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC41550.3628824129977406No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36350.31746752617251195No Hit
GTAATACACAGCCGAGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA35060.30620114078702254No Hit
CCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT33920.29624480021379934No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCGTCTATGATTA32190.281135616712329No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31180.2723146483097365No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30510.26646311481494744No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC29970.2617469534907891No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG29830.2605242449993406No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT29510.2577294827331727No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC26890.23484736667892286No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26740.23353732186665666No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC26310.22978186007149348No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26110.22803513365513856No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25600.22358098129343346No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG25080.21903949261091057No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24930.21772944779864437No Hit
CTACTACTGTTACTCAACAGACAGCAGTGATAATCATAGACGAGTGTTCG24440.21344996807857475No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24020.20978184260422936No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23530.20550236288415974No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23520.20541502656334196No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22870.1997381657101884No Hit
GTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG22780.19895213882282864No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22630.19764209401056243No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGATAATCATAGACGAGTGTTCGGCGGAGGGACCGAGCTGACCGTC21970.19187789683659112No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21570.1883844440038812No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20890.1824455741882744No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20530.1793014666388355No Hit
GTGTATTACTGTGCGAGAGACATATATTACGATGTTTTGACTGGTTATGG19980.1744979689938594No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA19910.17388661474813516No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19810.1730132515399577No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGG19660.17170320672769146No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCGTCTATGATTAT19660.17170320672769146No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19080.1666377001202621No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18870.16480363738308942No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGT18680.16314424728755222No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18620.16262022936264572No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG18590.1623582204001925No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18440.16104817558792628No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17710.15467262416823072No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACA17650.15414860624332422No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA17310.1511791713355208No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG17160.14986912652325463No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17120.1495197812399836No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16440.1435809114243768No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16410.14331890246192355No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT16190.14139750340393312No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC16100.14061147651657338No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16090.14052414019575563No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16080.14043680387493787No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC15930.13912675906267166No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTG15320.13379924349278907No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15320.13379924349278907No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15210.13283854396379385No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15200.1327512076429761No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14620.12768570103554674No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACT14440.1261136472608273No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTC14390.12567696565673855No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGT13840.12087346801176245No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGATAATCATA13680.11947608687867849No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13620.11895206895377203No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA13600.11877739631213652No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13570.11851538734968328No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13510.11799136942477678No Hit
GCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG13450.1174673514998703No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC13350.11659398829169283No Hit
GGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGG13250.11572062508351534No Hit
CCTCATAGATGACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGGCAATGCAT13040.11388656234634267No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12900.11266385385489419No Hit
CAGTAATACACAGCCGAGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA12780.11161581800508123No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12600.11004376423036177No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12490.10908306470136656No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12480.10899572838054881No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12440.10864638309727782No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12340.10777301988910033No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12070.10541493922702116No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT12060.10532760290620341No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC11990.10471624866047917No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11950.10436690337720818No Hit
TCATAGACGAGTGTTCGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT11940.10427956705639044No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11790.10296952224412423No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11790.10296952224412423No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC11690.10209615903594677No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11630.10157214111104026No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11570.10104812318613378No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11530.1006987779028628No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG11510.1005241052612273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAA205.153783E-4101.1607570
GTGGTAT2050.046.70081
AGGGGGG1600.046.3653470
TGGTATC2150.046.1169622
ATCTCGT1800.043.9446737
TCTCGTA1800.042.0617638
GGATGGG7100.041.7941170
GGTATCA7700.041.740461
TCGTATG1950.040.66617240
CTTGAAA2050.039.56794457
TAGCGCT2550.038.9322529
GTATCAA18600.038.235831
CTAGCGC2600.038.170228
TATACAC2600.038.135183
TAACCCA451.056202E-437.9890675
CGTATGC2200.037.6387241
GTATGCC2300.037.56527742
ACGAGAC2850.037.17955422
ACTAGCG2650.036.1459827
CACGAGA2850.035.98021321
Produced by FastQC (version 0.11.5)