FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144999
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC63660.5559830183257802No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC40180.3509173370457092No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCG38580.33694352571486963No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38240.3339740908070662No Hit
CCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT30360.26515307000268123No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29110.2542360299004628No Hit
GTAATACACAGCCGAGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA28710.2507425770677529No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28460.2485591690473092No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28010.24462903461051058No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT27110.2367687657369133No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC26480.23126657752539523No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCGTCTATGATTA26290.22960718742985803No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG26020.22724910676777885No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC25750.22489102610569967No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC24480.21379931336184574No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24110.21056786949158907No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23870.20847179779196315No Hit
GTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG23540.20558969920497747No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23280.20331895486371604No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23250.2030569459012628No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG23120.20192157373063205No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22750.19869012986037543No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACA22110.19310060532803958No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21590.18855911664551672No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGATAATCATAGACGAGTGTTCGGCGGAGGGACCGAGCTGACCGTC21010.18349361003808737No Hit
CTACTACTGTTACTCAACAGACAGCAGTGATAATCATAGACGAGTGTTCG20620.1800874935261952No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20500.17903945767638224No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20390.17807875814738702No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20340.1776420765432983No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19910.17388661474813516No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19880.1736246057856819No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19660.17170320672769146No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18680.16314424728755222No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18570.16218354775855698No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA18400.1606988303046553No Hit
GTGTATTACTGTGCGAGAGACATATATTACGATGTTTTGACTGGTTATGG18260.15947612181320683No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17980.1570307048303099No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGG17760.15510930577231943No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGT16810.14681235529463346No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16740.1462010010489092No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG16730.14611366472809145No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG16640.14532763784073172No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC16400.1432315661411058No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16310.14244553925374606No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15980.1395634406667604No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCGTCTATGATTAT15890.13877741377940067No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15480.13519662462587304No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15410.1345852703801488No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15130.13213985339725187No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA14990.13091714490580342No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14620.12768570103554674No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14590.1274236920730935No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC14550.12707434678982255No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTC14480.1264629925440983No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14310.12497827509019659No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14110.12323154867384165No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13960.12192150386157544No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTG13920.12157215857830443No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA13860.12104814065339795No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT13680.11947608687867849No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13620.11895206895377203No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13440.11738001517905255No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGATAATCATA13420.11720534253741707No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC13270.11589529772515084No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCGTCTATGATTATCACTGCTGTCTGTTGAGTAACAGT13210.11537127980024438No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12970.11327520810061843No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGT12490.10908306470136656No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12430.10855904677646007No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12390.10820970149318908No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12160.10620096611438087No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12100.10567694818947442No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11970.10454157601884367No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT11930.10419223073557271No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC11710.10227083167758226No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11620.10148480479022252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2700.049.3138472
GTGGTAT2800.048.7953951
GTAATAG306.6301617E-445.5423661
GGTATCA7850.040.4659921
CGGGGTT350.001423024638.995396
GGCCAAG9500.038.61861470
GTATCAA19950.037.32421
GCTTATC557.919338E-637.261941
GCTTGTG853.274181E-936.1661
ATTCAAC400.00267456934.29172534
TATCAAC21800.034.1403542
ATCAACG22350.033.44423
GGATGGG8850.032.6856870
TCAACGC22900.032.635254
ACGTACC851.32688E-732.12648413
ATACGTA851.3314639E-732.11385311
AACGCAG23450.032.0111436
CAACGCA23350.032.0021135
GACAGCC2050.031.6575011
GACAGGT1307.2759576E-1231.5293351
Produced by FastQC (version 0.11.5)