FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477278
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45890.3106388912581112No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38520.2607498385544224No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36670.2482268063289374No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATATCAAATTGTTCCTGCCTGCATATGAGCTGCAGTAATAAT35810.24240528864573901No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATATCAAATTGTTCCTGCCTGCATATGAGCTG35140.23786992021813091No Hit
GATTATTACTGCAGCTCATATGCAGGCAGGAACAATTTGATATTCGGCGGAGGGACCAAACTGACCGTCCTAGGT31800.21526077014617423No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31060.21025155725598027No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30090.2036854268458611No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG29750.2013838965990152No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC29690.200977744202513No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAAA29090.19691622023749084No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27530.18635625792843324No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25770.17444245429770158No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25180.17044862239876313No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT23390.15833174256978036No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23370.15819635843761296No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23280.15758712984285964No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCAGCTCATAT22950.15535329166209744No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21910.14831331678939239No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC21470.14533486588170946No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21450.14519948174954206No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21200.1435071800974495No Hit
CCACCACACCCTCCAAACAAAGCAACAACAGGTACGCGGCCAGCAGCTAT21030.14235641497402654No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACCTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20390.1380241227446696No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20280.13727951001774888No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19940.13497797977090298No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19860.13443644324223333No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG19600.13267644952405708No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19570.13247337332580597No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19140.12956261448420675No Hit
GGCTCAGATAGCTGCTGGCCGCGTACCTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19110.12935953828595567No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTA18520.1253657063870172No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGGAGAGC18290.12380878886709204No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17570.11893496010906546No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17080.11561804887096404No Hit
ATATATTACTGTGCGAAAGACTCAGATTACGATTTTTGGAGTGGTTCCCGATTCGACCCCTGGGGCCAGGGAACC17060.11548266473879662No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16500.11169190903810929No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16180.1095257629234308No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16150.1093226867251797No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16010.10837499780000784No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT16000.10830730573392416No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15890.10756269300700341No Hit
GTTTGGTCCCTCCGCCGAATATCAAATTGTTCCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCGTCAG15680.10614115961924567No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15650.10593808342099456No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15620.10573500722274344No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15550.10526116276015753No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15340.10383962937239978No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT15250.10323040077764645No Hit
GGGTACTGTTGGTACCAGGAGACATAGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGA15250.10323040077764645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT300.00216786670.4571270
ACCGATA200.00663574753.12244856
GTCGTAT759.0221874E-1041.2607839
GGTATCA13000.041.048681
CGTATGC3150.040.52633341
TGGTATC4500.040.0319062
AGGGGGG3550.039.6941570
GTATCAA34850.039.207231
TGTCGTA801.717126E-938.63302238
TCGTATG3050.038.3980740
CACGAGA4000.037.38702421
GTGGTAT4900.036.7639921
ATGCCGT3450.036.178844
TATACAC4500.035.4999923
ACGAGAC4350.035.1602122
TCTCCGA4400.034.75242211
CGAGACA4900.034.6841923
TATCAAC40600.033.5707632
ATCAACG40950.033.3668373
ATACACA5400.032.7304884
Produced by FastQC (version 0.11.5)