FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477278
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42320.2864728236662294No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39000.2639990577264401No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39000.2639990577264401No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATATCAAATTGTTCCTGCCTGCATATGAGCTG33890.22940841195766812No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33770.22859610716466366No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATATCAAATTGTTCCTGCCTGCATATGAGCTGCAGTAATAAT33440.22636226898390147No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG29470.19948851874867154No Hit
GATTATTACTGCAGCTCATATGCAGGCAGGAACAATTTGATATTCGGCGGAGGGACCAAACTGACCGTCCTAGGT28800.19495315032106347No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26760.18114396883998812No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAAA26640.1803316640469837No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26610.1801285878487326No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC26400.17870705446097485No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25840.1749162987602875No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25330.17146400339001866No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24630.1667255587641595No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23300.15772251397502704No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC23120.1565040567855204No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22450.15196868835791233No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT21300.14418410075828653No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20610.13951334819851105No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACCTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20460.13849796720725552No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20430.1382948910090044No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20390.1380241227446696No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCAGCTCATAT20320.13755027828208366No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19120.12942723035203937No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18550.1255687825852683No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18490.12516263018876608No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18400.12455340159401276No Hit
GGCTCAGATAGCTGCTGGCCGCGTACCTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17670.11961188076990248No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGGAGAGC17490.11839342358039584No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17380.11764881085347512No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTA17120.11588881713529885No Hit
GTTTGGTCCCTCCGCCGAATATCAAATTGTTCCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCGTCAG16420.11115037250943967No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16290.11027037565035153No Hit
CCACCACACCCTCCAAACAAAGCAACAACAGGTACGCGGCCAGCAGCTAT16210.1097288391216819No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16040.10857807399825897No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15960.10803653746958934No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG15940.10790115333742192No Hit
GGGTACTGTTGGTACCAGGAGACATAGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGA15900.10763038507308711No Hit
ATATATTACTGTGCGAAAGACTCAGATTACGATTTTTGGAGTGGTTCCCGATTCGACCCCTGGGGCCAGGGAACC15770.106750388213999No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15660.10600577548707825No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15620.10573500722274344No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14930.10106425466296796No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14870.10065810226646575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5300.045.496531
TGGTATC5650.042.656472
GGTATCA16150.039.1143571
GTATCAA37750.036.7961271
GTGCTTA800.001954088236.55513470
TCTGGAG12450.034.64663370
ATCAACG43850.031.4936773
TATCAAC44800.030.9899122
ATATGGC9650.030.9350626
TCAACGC44600.030.7316744
TATGGCC9550.030.548567
CAACGCA45000.030.3046445
AACGCAG45600.029.9058956
CCGTGTC9900.029.81424312
CGGCTCT10050.029.72091721
ATGGCCG9750.029.573998
TGGCCGT10050.028.6911839
AAACCGT10250.028.57620848
GTGTATA1551.8189894E-1228.4844231
CGCAGAG48600.028.0598558
Produced by FastQC (version 0.11.5)