FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077141
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35460.329204811626333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30470.28287847180638376No Hit
GAGTTACACTACCCCTGCACTCACTTTTGGCGGAGGGACCAAGGTGCAGGTCAAGCGAACTGTGGCTGCACCAT29580.274615858091002No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25890.2403585045968912No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC25290.23478820321573501No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24530.2277324881329371No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23820.22114096483190224No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22990.21343538125463613No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19580.18177750173839824No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19280.1789923510478201No Hit
GACCAGGGGAAGCCCCTAAACTCCTGATCTTTGCTACATCCAGTCTGCAAAGTGGGGTCCCATCAAGATTCAGTG18680.17342204966666389No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18290.16980135376891234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18180.1687801318490337No Hit
GTCTATTACTGTGCGAGACAGGAATACTGGAAAACGCATAGTAGTGGTTTTTATCCCCTCGCGAGAGACGTCTA17880.1659949811584556No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGATAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAC16820.15615411538507956No Hit
GTAATAGACAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGAGACA16590.1540188331889697No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT15790.1465917646807614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15680.14557054276088274No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15330.14232120028854162No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15270.141764170150426No Hit
GTGTTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACTCTATCTCCTACAGATGCAGACAGGGAGGATGGAGA15260.14167133179407337No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15250.14157849343772078No Hit
GTGCAGGGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCA15200.14111430165595776No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15190.14102146329960516No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14870.13805063589632183No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14720.1366580605510328No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14620.13572967698750674No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14460.13424426328586508No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14410.13378007150410207No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14150.13136627423893438No Hit
GTGCAGCCACAGTTCGCTTGACCTGCACCTTGGTCCCTCCGCCAAAAGTG14100.13090208245717133No Hit
CCCCTGGTCTCTGCTGATACCAATTTAAGTAGGTGTTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACTCTAT13750.12765273998483023No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13400.12440339751248908No Hit
GGGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTG13390.12431055915613648No Hit
GTCCCAGACCCACTGGCACTGAATCTTGATGGGACCCCACTTTGCAGACT13100.12161824682191097No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13010.12078270161473754No Hit
GTAGGAGATAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACACCTACTTAAATTGGTATCAGCAGAGA12830.11911161120039067No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12340.11456253173911307No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12300.11419117831370267No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12240.11363414817558704No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGGCA12160.11289144132476622No Hit
CTCCTGTACAGCTTCTGGATTCAAGATCAGCAGTTATGACATAACGTGGGTCCGCCAGACTCCCGGGAAGGGG12090.11224157283029798No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11950.11094183584136152No Hit
GTTTAGGGGCTTCCCCTGGTCTCTGCTGATACCAATTTAAGTAGGTGTTA11870.11019912899054071No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11820.1097349372087777No Hit
GCATTAACACCTACTTAAATTGGTATCAGCAGAGACCAGGGGAAGCCCCT11630.1079710084380782No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC11590.1075996550126678No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11590.1075996550126678No Hit
GTCTGCATCTGTAGGAGATAGAGTCACCATCACTTGCCGGGCAAGTCAGA11470.10648559473643655No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCTGCACTCACTTTTGGCGG11230.10425747418397406No Hit
GAGATAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACACCTAC10990.10202935363151155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)