FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627052
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA69100.42469447811133265No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCCAACGCCAGCGCCCCAGGTCTGA46920.28837431133116825No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC45640.2805073224457485No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41110.2526655570934426No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCCAACGCCAGCGCCCCAGGTCTGACAGTAATAGT39010.23975877845330082No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT37670.231523024463877No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC36710.22562278279981218No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34970.2149285947836947No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCCTGGT34930.2146827513810253No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31980.19655180043415946No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG30720.18880773325007436No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC30250.1859190732687093No Hit
GACTATTACTGTCAGACCTGGGGCGCTGGCGTTGGGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT30110.18505862135936652No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29870.18358356094335032No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT29250.17977298820197513No Hit
CAGTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATA28860.17737601502594877No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28080.17258206867389608No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27610.16969340869253102No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26940.1655755316978191No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26460.16262541086578672No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC26390.16219518491111531No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG26260.16139619385243986No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26250.16133473300177253No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26090.16035135939109504No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25960.15955236833241962No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGTCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA25380.1559876389937138No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25020.15377504836968947No Hit
CTGTTAACTTTCATCAAGAATCGAGGGCCCTTCTCTGGCAGTTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC24570.1510093100896591No Hit
CACCAGGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG24460.15033324073231832No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23830.1464612071402758No Hit
GGACTGGAGTGGGTCTCAACTTTCAGTGGTAGTACTGAATACTACGCAGACTCCGTAAAGGGCCGCTTCACCAT23730.14584659863360236No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23620.1451705292762616No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23490.14437153821758617No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23420.1439413122629148No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23120.14209748674289452No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22940.14099119143088235No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC22920.14086826972954766No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC22830.14031512207354158No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA22590.13884006165752538No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC21890.13453780211081146No Hit
CTATTACTGTCAGACCTGGGGCGCTGGCGTTGGGAGGGTGTTCGGCGGAG21680.13324712424679727No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21640.1330012808441279No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21270.13072722936943626No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC21230.1304813859667669No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20900.1284531778947446No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGACATAGCTGC20630.12679373492672638No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT20360.12513429195870815No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20200.12415091834803067No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT20010.12298316218535119No Hit
ATATATTACTGTGCGAAAGATATCGGGGGGTACAGCTATGGTTACTTTGA19710.12113933666533092No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG19390.11917258944397598No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC19390.11917258944397598No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT19280.11849652008663521No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT19230.11818921583329851No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19210.11806629413196382No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG18980.11665269456661495No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18770.11536201670260078No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGTAACCATAGCTGTACCCCCCGATATCTTT18770.11536201670260078No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT18360.11284212182523976No Hit
CCCCTGGTGTGGCTGCCATCACTGTTAACTTTCATCAAGAATCGAGGGCC18310.11253481757190305No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18180.11173582651322761No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18180.11173582651322761No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT18090.11118267885722154No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC18090.11118267885722154No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18070.11105975715588684No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17950.11032222694787874No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCGCTGGCGTTG17860.10976907929187267No Hit
GTATTCAGTACTACCACTGAAAGTTGAGACCCACTCCAGTCCCTTCCCTG17850.10970761844120532No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGTCATGAGCTGGGTCCG17770.1092159316358666No Hit
GTATCAACGCAGAGTACGGGAAGGAAGTCCTGTGCGAGGCAGCCAACGGC17650.10847840142785849No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC17290.10626581080383417No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACT17070.10491367208915266No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC17000.10448344613448125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG600.062.9805835
GAACCGA252.4097218E-455.91316651
AGTATCG1200.051.4882134
GTGGTAT3450.050.5236631
GGTATCA14850.046.9512861
CGTATGC2450.045.09076341
TCGTATG2550.043.2835340
TCTCGTA2400.043.0237138
TCTGGAG23250.042.8107670
GACCGAT4900.042.58186726
TGGTATC4050.042.1934852
ACCGATC4900.041.8941127
CTCTTAT1900.041.3730931
ACGAGAC5200.040.75292622
TCAGTAT4950.040.175132
TATCGCG602.996876E-740.1020936
ATGCCGT2250.040.01828444
TGCCGTC2350.039.82253345
GGATGGG12150.039.52871370
GTATCAA38450.039.3777731
Produced by FastQC (version 0.11.5)