FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032808
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33650.3258107992966747No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAC32690.3165157512335304No Hit
GATTACTACTGCACCTCATATGGAAATACCATCGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG30200.2924067203197496No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29710.28766237287085306No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28770.27856097164235755No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24580.23799195978342538No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACGATGGTATTTCCATATGAGGTGCAGTAGTAATCAG24390.23615231485426139No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23920.2316016142400136No Hit
GTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG23850.23092385031874268No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG23800.23043973323212058No Hit
GTAGTACACGGCCGCGTCCGCAGCGGTCACAGAACTCAGTTTCAGGGAGAACTGATTCTTGGACGGGTCCACTGA23160.22424303452335767No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20980.20313552954663405No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20050.19413095173546294No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19810.19180718971967683No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGCACCAGCAGTGACATT19500.18880566378261981No Hit
CTCCTGCACTGGCACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC19290.18677237201880698No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19200.1859009612628872No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18960.1835771992471011No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC18280.17699320686904052No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18120.17544403219184979No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17940.1737012106800102No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17930.1736043872626858No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGCACCTCATAT17310.16760133538857175No Hit
GTGCAGTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG17160.16614898412870543No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16930.16392204553024378No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16920.16382522211291936No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16800.1626633411050263No Hit
CTACTGCACCTCATATGGAAATACCATCGTGTTATTCGGCGGAGGGACCA16070.1555952316403436No Hit
GTCAGTCACCATCTCCTGCACTGGCACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA15990.15482064430174824No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15930.15423970379780172No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT15910.15404605696315288No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15870.1536587632938552No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15750.15249688228596217No Hit
GGCTCAGATAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15420.14930170951425628No Hit
GGATGAGGCTGATTACTACTGCACCTCATATGGAAATACCATCGTGTTATTCGGCGGAGGGACCAAGCTGACCGT14810.14339548105746663No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14790.1432018342228178No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14250.1379733696872991No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAT13600.13167984756121176No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13280.12858149820683032No Hit
GTGTACTACTGTGCGAGAGGACGGATGTGGTACTTCGATATCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCA13220.1280005577028838No Hit
CCCCTGAGGGCCGGCTAGTGACGTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA12930.12519267860047562No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12870.12461173809652908No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12640.12238479949806741No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12630.12228797608074299No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12290.11899597989171269No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT11730.11357386852154515No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11680.11308975143492303No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT11670.11299292801759861No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11630.11260563434830094No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11480.11115328308843463No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGC11390.11028187233251485No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT11300.10941046157659506No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11170.10815175715137762No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC10900.10553752488361824No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10860.10515023121432057No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10530.1019550584426147No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC10480.1014709413559926No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10430.10098682426937049No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10420.10089000085204607No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTATGACGTCACTAGC10380.1005027071827484No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10360.10030906034809955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3700.044.0499270
GGTATCA13500.042.043211
GTATCAA35650.040.8034971
GTGGTAT4250.040.784431
TGGTATC4150.039.310292
TAGCTCG350.001387307439.197236
TCGTATG3050.038.45504440
TCGTATT451.0020939E-438.32937640
CTTATAC4200.037.2238851
CGTATGC3300.036.65377841
ACGAGAC4050.036.0884822
GCCGTCT3100.035.95733346
GAGCCTA4200.035.76476331
TCTCGTA2900.035.5688638
AGCCTAT3850.035.49853532
TATCTCG3000.035.44080436
CACGAGA4150.035.21717521
TTATACA4450.035.1326562
TATCAAC42050.034.5932122
CGTATTC400.002574429834.55927741
Produced by FastQC (version 0.11.5)