FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032808
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44910.43483396720397205No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30670.2969574209339974No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29490.2855322576897158No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAC29370.2843703766818228No Hit
GATTACTACTGCACCTCATATGGAAATACCATCGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG26740.2589058179255002No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG26430.25590429198844317No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26280.25445194072857685No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACGATGGTATTTCCATATGAGGTGCAGTAGTAATCAG22990.22259703642884252No Hit
GTAGTACACGGCCGCGTCCGCAGCGGTCACAGAACTCAGTTTCAGGGAGAACTGATTCTTGGACGGGTCCACTGA21610.20923540483807251No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21160.20487835105847363No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20950.2028450592946608No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20480.198294358680413No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20390.19742294792449325No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20220.19577694982997806No Hit
CTCCTGCACTGGCACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC19910.19277542389292104No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19820.19190401313700126No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19600.189773897955864No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGCACCAGCAGTGACATT18090.17515356193987655No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17450.1689568632311136No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17340.16789180564054498No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16640.16111416642783558No Hit
GTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG16560.1603395790892402No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16480.15956499175064484No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16380.15859675757740063No Hit
GTCAGTCACCATCTCCTGCACTGGCACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA15670.15172229494736678No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGCACCTCATAT15520.1502699436875005No Hit
GGCTCAGATAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15280.1479461816717144No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15180.14697794749847018No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15120.14639700699452365No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14920.14446053864803526No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14890.144170068396062No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14850.14378277472676432No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14160.1371019589313793No Hit
CTACTGCACCTCATATGGAAATACCATCGTGTTATTCGGCGGAGGGACCA14150.13700513551405488No Hit
GGATGAGGCTGATTACTACTGCACCTCATATGGAAATACCATCGTGTTATTCGGCGGAGGGACCAAGCTGACCGT13860.1341972564116467No Hit
GTGCAGTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG13680.13245443489980713No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12970.12557997226977327No Hit
CCCCTGAGGGCCGGCTAGTGACGTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA12440.120448331151579No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12260.11870550963973941No Hit
GTGTACTACTGTGCGAGAGGACGGATGTGGTACTTCGATATCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCA12180.11793092230114406No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12150.11764045204917081No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAT12010.11628492420662892No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11830.11454210269478936No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11770.11396116219084282No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC11690.11318657485224747No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11630.11260563434830094No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11470.1110564596711102No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11420.11057234258448811No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11210.10853905082067528No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT11180.10824858056870201No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT11000.10650575905686245No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11000.10650575905686245No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT10800.10456929071037405No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10750.10408517362375194No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10620.10282646919853447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)