FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420320
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTTTCATTTTCGGCCCTGGGA32870.2314267207389884No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30610.2155148135631407No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27050.19045003942773459No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26730.18819702602230484No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24370.17158105215726033No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23930.1684831587247944No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21790.15341613157598286No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20580.1448969246367016No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20490.14426326461642447No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20020.1409541511771995No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18980.1336318576095528No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCT18810.1324349442379182No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18810.1324349442379182No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC18450.12990030415680973No Hit
GTATGGTAGCTCACCTTTCATTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT18360.1292666441365326No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAATG18280.12870339078517518No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18210.1282105441027374No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17090.12032499718373324No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17070.1201841838458939No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC16970.11948011715669708No Hit
GTAATATACGGCCGTGTCCTCGGCTGTCAGGCTGTTCATTTGCAGATACA16800.11828320378506252No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC16660.11729751042018699No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTTTCATTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA16430.11567815703503435No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA16260.11448124366339979No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16010.1127210769404078No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15340.10800383012278922No Hit
CATCTATGGTGCATCCAGGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15210.1070885434268334No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15150.10666610341331531No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15050.1059620367241185No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14410.10145600991325898No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14260.10039990987946379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.042.1845051
GTATCAA47050.037.4927521
CGTATGC2600.037.15125741
ATCGCGT400.00269009534.25181637
TATCAAC51700.034.1193772
GAGCGAT3500.034.0782232
ATCTCCG4100.033.8490810
TCTCCGA4200.033.04314811
ATCAACG53950.032.7580223
TCAACGC54150.032.6358834
TGAGCGA4100.032.3802931
CAACGCA54650.032.3372965
CTTATAC4650.032.038261
GTCACCG6900.031.85480770
ACGAGAC4150.031.82566522
AACGCAG55650.031.8170476
GATGTCG1305.456968E-1231.5833136
CCCACGA4100.031.3778419
CGAGACA4650.030.59490623
GTCGTAT1251.3096724E-1030.22450339
Produced by FastQC (version 0.11.5)