FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420320
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35820.25219668807029405No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTTTCATTTTCGGCCCTGGGA29200.2055874732454658No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27180.19136532612369042No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26010.18312774586008787No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23470.16524445195448914No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22800.16052720513687055No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22570.1589078517517179No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21650.15243043821110736No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20590.14496733130562128No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20580.1448969246367016No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18660.13137884420412302No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18550.13060437084600654No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17820.1254646840148699No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAATG17770.1251126506702715No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC17250.12145150388644813No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCT17190.12102906387293005No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16710.1176495437647854No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16620.11701588374450828No Hit
GTATGGTAGCTCACCTTTCATTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT16550.11652303706207051No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15880.11180579024445195No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC15800.1112425368930945No Hit
GTAATATACGGCCGTGTCCTCGGCTGTCAGGCTGTTCATTTGCAGATACA15630.11004562352145997No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTTTCATTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA15400.10842627013630732No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15030.10582122338627915No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14600.1027937366227329No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14430.10159682325109834No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC14400.1013856032443393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.038.576341
GTATCAA49550.037.9094471
TATCAAC55500.033.6564032
ATCAACG55450.033.607963
TCAACGC55850.033.41844
CAACGCA57000.032.682555
AACGCAG58450.032.2755436
CGCAGAG60650.030.8818158
AGAGTAC61500.030.39680911
CGATTGC603.976007E-430.3147163
GCAGAGT62850.029.8535699
ACGCAGA67400.028.0398547
CAGAGTA68000.027.64231710
GCGTGTT6650.026.44397251
AGTACGG49500.026.3664613
GAGTACG49900.026.28877412
AATGGTA12300.026.18764370
ACGGGAA6850.026.17394416
ACGGGAT3200.025.3714416
TCTGGAG11550.024.92131670
Produced by FastQC (version 0.11.5)