FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202961
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38560.3205423949737356No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGACTGTTCATTTGCAAATACA34800.28928618633521785No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC29180.24256812980636946No Hit
GTATATTACTGTGCGAAGGATCGTCGGCCCGTTACGTCGTACTTCCTTGGCTCCTGGGGCCAGGGAACCCTGGT26110.21704776796587752No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGACCTGGCTTTTT23040.19152740612538563No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22360.18587468754182387No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18160.1509608374668838No Hit
GGGTATTAGGAACTGGTTAGTCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATC17420.1448093495965372No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17230.1432299135217185No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16820.13982165672868863No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16040.13333765600048547No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16020.1331713995715572No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGAC15870.13192447635459503No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGGAACTGGTTAGTCTGGTATCAGCAGAAA15420.1281837067037086No Hit
GATCTATGGTGCATCCAGTTTGGAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGAACTGGGACAGATTTCA15390.12793432206031616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15170.12610550134210502No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14770.12278037276353931No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14430.11995401347175844No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14040.11671201310765686No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14010.11646262846426443No Hit
GGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13710.11396878203034012No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13540.11255560238444971No Hit
GAGTGAAATCTGTCCCAGTTCCACTGCCGCTGAACCGTGATGGGACCCCA13160.10939673023481226No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT13040.10839919166124255No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12900.10723539665874456No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12830.10665349915749554No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT12570.10449216558142782No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12490.1038271398657147No Hit
GTCTGAGAGTCGAGGACACGGCCGTATATTACTGTGCGAAGGATCGTCGGCCCGTTACGTCGTACTTCCTTGGCT12430.10332837057892982No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12340.10258021664875253No Hit
GAGTTTGGGCTGACCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT12300.10224770379089596No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12160.10108390878839796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAA200.007156611452.11643245
GTGGTAT2300.045.8991051
AATACCG404.7980982E-543.4303645
GTATCAA26500.041.3790321
ACGGGTA1150.038.4527916
TATCAAC30600.035.7026822
ATCAACG31250.035.2647823
TTTCGAT800.002368091435.1504870
ATACCGC501.7738347E-434.78418446
TCAACGC31750.034.6906784
CAACGCA32150.034.2590685
AACGCAG32550.034.151386
GCCGTTA951.0104486E-832.2229415
ACGCAGA34500.032.221087
CGCAGAG35150.031.7219588
AGAGTAC35300.031.48955211
GCAGAGT36350.030.7682619
CAGAGTA36200.030.7079410
TCTGGAG16100.029.25567870
CGCGTGT500.007211881727.99883750
Produced by FastQC (version 0.11.5)