FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086719
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGCCTGAGTGGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT31860.29317606483368747No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC27670.25461963948361993No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG27000.24845429223193852No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC26920.2477181313660661No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT26910.24762611125783207No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21880.20133999681610426No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21430.19719909194557192No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21320.19618687075499738No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT20680.19029758382801812No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19540.17980729148933625No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19130.17603446705174014No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCCACTCAGGCTGTCATCCCATGTTGCA18880.17373396434588886No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG18760.17262972304708024No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18550.17069730077416517No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG18180.16729255676950527No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17740.16324367200720702No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17530.16131124973429192No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17520.1612192296260579No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17340.15956286767784497No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCCACTCAGGCTGTCATCCCATGTTGCACAGTAATAAT17320.15937882746137685No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC17070.15707832475552558No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16290.14990075631326957No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15920.14649601230860967No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15910.14640399220037562No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15670.1441955096027584No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15550.14309126830394978No Hit
GTCCTGGGCCCAGTCTGTATTGACTCAGCCACCCTCAGCGTCTGGGACCC15190.13977854440752394No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG15140.13931844386635367No Hit
GTATTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15120.13913440364988558No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14900.13710996126873642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.13471743845465112No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14310.1316807748829274No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA13760.12661966893005458No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13700.12606754828065028No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13570.12487128687360763No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13300.12238674395128822No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12920.11888997983839429No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12800.11778573853958568No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC12750.1173256379984154No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGGTTCAGGGAGA12660.11649745702430896No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12650.1164054369160749No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTATTGACTCAGCCACCCTCAGCGTCT12560.11557725594196844No Hit
GCCCCAAGGACGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCAT12520.11520917550903223No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11850.1090438282573508No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11770.10830766739147839No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11070.10186625981509478No Hit
GTATATTACTGTGCGAGAATGGGATGGGAATTTTGTAGTGGTGGTAGCTGCTACCTTCCATTCGACCCCTGGGGC11010.10131413916569047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4100.051.4743161
TCGTATG900.049.77286540
GGTATCA18700.044.0510751
TGGTATC4800.043.260482
GACGGAT4100.042.9056670
TCTCGTA903.6379788E-1241.99810438
CGTATGC1250.038.64533241
AGACGGA1350.037.83033425
GCTACGG750.001717243937.5281570
GTATCAA42300.036.2926671
ATCTCGT1051.8189894E-1135.95160337
CGAGACG1450.035.21323823
CATCTCG1300.034.2935736
CTACATC1400.034.20792433
TATACAC1900.034.041033
CACGAGA1700.034.03946321
CTTATAC1700.034.0394631
CCCACGA1800.034.037919
CCGTCTT1650.033.73457747
GCCGTCT1550.033.6270246
Produced by FastQC (version 0.11.5)