FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086719
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGCCTGAGTGGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT29320.26980295734223847No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23690.21799563640646755No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22810.209897866881871No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC22600.20796544460895597No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21860.20115595659963614No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21160.19471454902325255No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20050.18450031700927289No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19150.17621850726820826No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17930.164992054063654No Hit
GTATTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17380.15993094811078118No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17220.15845862637903635No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16990.15634216388965316No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16940.1558820633484829No Hit
GTCCTGGGCCCAGTCTGTATTGACTCAGCCACCCTCAGCGTCTGGGACCC16740.1540416611838019No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16420.15109701772031223No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16400.15091297750384414No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16310.1500847965297377No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15540.14299924819571572No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15150.13941046397458773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14750.13572965964522568No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14370.13223289553233172No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14170.13039249336765069No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14170.13039249336765069No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14150.1302084531511826No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTATTGACTCAGCCACCCTCAGCGTCT14090.12965633250177827No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13940.12827603087826753No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCCACTCAGGCTGTCATCCCATGTTGCA13670.12579148795594813No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13210.12155856297718177No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13170.12119048254424557No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCCACTCAGGCTGTCATCCCATGTTGCACAGTAATAAT13110.12063836189484126No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12870.11842987929722402No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12780.11760169832311758No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12780.11760169832311758No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12470.11474907496786196No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGGTTCAGGGAGA12200.11226453204554258No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA12010.11051614998909562No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG11770.10830766739147839No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC11730.10793958695854218No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11720.10784756685030812No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC11510.10591514457739305No Hit
GCCCCAAGGACGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCAT11430.10517898371152064No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11350.10444282284564822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG200.00781023350.97675
GTGGTAT5700.044.7906071
TGGTATC5650.044.563952
GGTATCA18750.042.8460961
GACGGAT3750.038.97827570
GTATCAA44500.037.9421771
TATCAAC52800.032.027492
AACGAAG751.8779483E-631.7173776
ATCAACG55550.030.4779433
AACGCAG55650.030.3495126
TCAACGC55400.030.3080234
CAACGCA56000.029.9791565
CGCAGAG58850.028.8147348
ATAGCGG2250.028.80610563
ACGCAGA58750.028.8059377
ACGAACC2350.028.3832552
AGAGTAC60100.028.10233511
CAGAGTA60400.027.96275310
GCAGAGT60500.027.9165369
GACAGGT2600.027.4945411
Produced by FastQC (version 0.11.5)