FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589312
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74551.2650344808861858No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG20600.34956016507384885No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT13090.22212342528236317No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10700.18156765855777585No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC10210.17325287793223285No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG10200.17308318853171156No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC9790.16612592311033883No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC9600.1629018245004344No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC9040.1533992180712424No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC8270.14033313423110338No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG7800.13235773240660295No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT7370.12506108818418768No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT7040.11946133796698524No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCC6970.11827351216333623No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG6390.10843152693310165No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG6310.10707401172893136No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA5960.10113488271068635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTC150.001929230972.5570857
TGAGTAC350.001575016338.1952554
GTATCAA47550.037.5772131
GGTATCA19500.037.064061
ATTAGCG502.2376634E-433.43219816
GTGGTAT5650.032.5722771
CAAACGC500.004719671330.53361967
AACGCAG60550.030.0237776
TTAGCGC450.005382106629.7250817
CGCAGAG61450.029.4752838
ATCAACG61750.029.1821123
CAACGCA62100.029.1667655
TCAACGC61550.029.15844
ACGCAGA62550.029.1172127
GCAGAGT62100.029.1129539
AGAGTAC61850.029.06113611
CCGAAAC1103.3832293E-728.86590868
CAGAGTA62250.028.8256210
TGGTATC6700.027.9528262
CGGGATA855.6376084E-627.53941517
Produced by FastQC (version 0.11.5)