FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136110
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT13490.9911101315112777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.2689001542869738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.24759385790904415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.24538975828374107No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.242450958783337No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC3010.22114466240540737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.20571596502828596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.1976342664021747No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.19469546690177064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.19249136727646757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.18514436852545735No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.17926676952464918No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.17265447064873998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.16604217177283082No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.1653074718977298No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.15428697377121445No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.14253177576959813No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.138858276394093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.138858276394093No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.13665417676878996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.12857247814267872No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.12416427889207259No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.11240908089045626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1520.11167438101535522No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.11093968114025421No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.10579678201454706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3150.086.3705570
GTTTCCT200.001375118778.9521368
TCAAATC150.001504730277.2010744
TATGACG150.001585236376.19459543
AAGGGGG2550.059.6342169
AAATCCC200.004266908459.3526846
GACTGAC150.00437491458.99027626
CGTGATA500.00456064958.30012570
GCGACTA200.004706831657.90080644
AAAGGGG2450.055.85729268
GCTTGAA3700.053.879256
TTGAAAA4050.053.61841658
AGCCATT200.00716668452.06742538
TCTTCTG3650.052.0329650
ACGTGAT453.428068E-451.988869
TGAAAAA4450.050.56932459
GTCTTCT3800.050.5628149
CTTGAAA4000.050.347157
AAAAGGG2500.050.28648867
CAGCTGA300.00876612249.4667766
Produced by FastQC (version 0.11.5)