FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580039
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52820.3342955458694374No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46620.2950560081111922No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43710.2766387411956287No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACCTCTGGATGATCACTACCACTGTCC43310.27410715811445163No Hit
GGATTACCTGTGGGGGAAGCAACATTGGAAGTCAAAGTGTGCACTGGTACCAGCAGAAGTCAGGCCAGGCCC41260.2611327948234189No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39680.25113304165276934No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACCTCTGGATGA37220.2355638057035301No Hit
GTGGTAGTGATCATCCAGAGGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC36920.23366511839264725No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35900.22720958153564563No Hit
GTCCCACACCTGACAGTAATAGTCGCCCTCATCCCCGGCTTCGACCCCGC33500.212020083048583No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32550.20600757323078733No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32020.20265322564822766No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCGCAACCTCACAGAGA31280.19796979694805No Hit
CCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAGTGCACACTTTGACTT30170.19094465389778353No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC29660.18771688546928272No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29580.18721056885304732No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29410.18613464604354701No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28300.17910950299328054No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28010.17727410525942713No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26580.16822369574421897No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26560.1680971165901601No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25910.1639832940832473No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25890.16385671492918844No Hit
GTGTGGGACAGTGGTAGTGATCATCCAGAGGTGCTATTCGGCGGAGGGAC25790.16322381915889417No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25490.16132513184801134No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACTTCCTATGTGGTGACT25190.1594264445371285No Hit
CCCCTGTGGTGGTCGTCTATAATGACAACGGCCGGCCCTCAGGGATCCCT24670.15613538653159825No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24610.15575564906942171No Hit
CTATTACTGTCAGGTGTGGGACAGTGGTAGTGATCATCCAGAGGTGCTATTCGGCGGAGGGACCAAGCTGACCGT24370.15423669922071545No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24330.15398354091259772No Hit
CACTACCACTGTCCCACACCTGACAGTAATAGTCGCCCTCATCCCCGGCTTCGACCCCGCTGATGGTCAGGGTGG24070.1523380119098326No Hit
CCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGGCAGACG23700.14999629755974378No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23340.14771787278668438No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23340.14771787278668438No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA22940.1451862897055073No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22620.14316102324056557No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT22460.14214839000809473No Hit
GGGTGGCCGTGTTCCCAGAGTTGGTGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGCCGTTGTCATTATAGA22030.13942693819582935No Hit
CCTCTCTCACTGCACAGGCTCTGTGACTTCCTATGTGGTGACTCAGCCAC22020.1393636486187999No Hit
CGGCCACCCTGACCATCAGCGGGGTCGAAGCCGGGGATGAGGGCGACTAT21780.13784469877009364No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC21360.13518653653485768No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21010.1329714013388277No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATAATGACAACGGCCGGCCCTCAGG20900.13227521599150402No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20830.131832188952298No Hit
GGCTCTGTGACTTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGGCAGACGGCCAGGATTAC20440.12936389544815033No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20430.1293006058711209No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20420.12923731629409146No Hit
CAGTAATAGTCGCCCTCATCCCCGGCTTCGACCCCGCTGATGGTCAGGGT19350.1224653315519427No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19170.121326119165413No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG19110.12094638170323643No Hit
CCCCAGGGCAGACGGCCAGGATTACCTGTGGGGGAAGCAACATTGGAAGT19040.12050335466403045No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18940.11987045889373617No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18910.11968059016264787No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18380.11632624258008821No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18300.11581992596385278No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18270.1156300572327645No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18260.11556676765573506No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG18110.11461742400029366No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT17920.11341492203673453No Hit
GCACAGGCTCTGTGACTTCCTATGTGGTGACTCAGCCACCCTCGGTGTCA17820.11278202626644027No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACTTCCTA17300.10949096826091001No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACTTCCTATGTGGTGACTCAGC17230.10904794122170401No Hit
GTGTATTACTGTGCGAGGGGCCGAGCTGGACTAATTGATGCTTTTGATAT17090.10816188714329203No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17080.1080985975662626No Hit
CCCTCGGTGTCAGTGGCCCCAGGGCAGACGGCCAGGATTACCTGTGGGGGAAGCAACATTGGAAGTCAAAGTGTG17060.10797201841220375No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC17030.10778214968111546No Hit
CTACCACTGTCCCACACCTGACAGTAATAGTCGCCCTCATCCCCGGCTTC16940.10721254348785061No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16890.10689609560270348No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16650.1053771457539972No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACCT16560.10480753956073237No Hit
CAGGTAATCCTGGCCGTCTGCCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCACCACATAGGAAGTCACAGAG16360.10354174802014382No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16250.10284556267282009No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16230.10271898351876124No Hit
ATCCAGAGGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT16230.10271898351876124No Hit
CTCACTGCACAGGCTCTGTGACTTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGGCAGACGG16200.10252911478767296No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16090.10183292944034926No Hit
CCTCTGGATGATCACTACCACTGTCCCACACCTGACAGTAATAGTCGCCC16010.10132661282411383No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15810.10006082128352527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4750.048.2459270
TCGTATG4150.044.03288740
ACGAGAC4600.043.52680222
CGTATGC4400.043.159341
TATACAC5050.043.019093
GTCGTAT405.080582E-543.01246339
CTTATAC4950.042.5181
TTATACA4950.042.5166552
ATGCCGT4600.042.2224244
CACGAGA4900.041.55315821
ATCTCGT4000.041.1611737
TCTCGTA4050.040.71814738
GGTATCA15150.040.5557371
GTATCAA39350.040.1138731
CCCACGA5000.039.36219819
ATACACA5850.038.296644
TACACAT5700.037.517885
GTATGCC5050.036.9752142
TCTACAT4900.036.90152432
ATCTCCG5450.036.72775710
Produced by FastQC (version 0.11.5)