FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580039
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49430.3128403792564614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45850.29018271067992624No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44480.28151203862689467No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42360.26809464829665597No Hit
GTGGTAGTGATCATCCAGAGGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC40440.25594304950700586No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACCTCTGGATGATCACTACCACTGTCC39690.2511963312297988No Hit
GGATTACCTGTGGGGGAAGCAACATTGGAAGTCAAAGTGTGCACTGGTACCAGCAGAAGTCAGGCCAGGCCC38710.24499395268091484No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACCTCTGGATGA33650.21296942670402438No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33510.21208337262561242No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30900.19556479302093177No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30850.19524834513578462No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30200.19113452262887182No Hit
GTCCCACACCTGACAGTAATAGTCGCCCTCATCCCCGGCTTCGACCCCGC29910.1892991248950184No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29470.18651438350572358No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCGCAACCTCACAGAGA29220.1849321440799879No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29190.18474227534889961No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28350.1794259508784277No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28120.17797029060675085No Hit
GGGTGGCCGTGTTCCCAGAGTTGGTGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGCCGTTGTCATTATAGA26750.16929961855371925No Hit
CCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAGTGCACACTTTGACTT26230.166008560548189No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26170.16562882308601243No Hit
GTGTGGGACAGTGGTAGTGATCATCCAGAGGTGCTATTCGGCGGAGGGAC25990.16448961069948273No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25280.15999605073039336No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24960.15797078426545166No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24220.15328735556527404No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACTTCCTATGTGGTGACT23660.14974313925162605No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22990.1455027375906544No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22920.14505971055144842No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22910.14499642097441898No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22890.14486984182036014No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22840.144553393935213No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA22640.14328760239462443No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22500.14240154831621246No Hit
CTATTACTGTCAGGTGTGGGACAGTGGTAGTGATCATCCAGAGGTGCTATTCGGCGGAGGGACCAAGCTGACCGT22070.13968009650394705No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT22030.13942693819582935No Hit
CCCCTGTGGTGGTCGTCTATAATGACAACGGCCGGCCCTCAGGGATCCCT21680.13721180299979938No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC20790.1315790306441803No Hit
GGCTCTGTGACTTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGGCAGACGGCCAGGATTAC20720.13113600360497432No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATAATGACAACGGCCGGCCCTCAGG20610.1304398182576506No Hit
CACTACCACTGTCCCACACCTGACAGTAATAGTCGCCCTCATCCCCGGCTTCGACCCCGCTGATGGTCAGGGTGG20500.1297436329103269No Hit
CCTCTCTCACTGCACAGGCTCTGTGACTTCCTATGTGGTGACTCAGCCAC20410.12917402671706205No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG20230.12803481433053235No Hit
CCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGGCAGACG19760.12506020421014924No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19720.12480704590203152No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19460.12316151689926642No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19190.12145269831947186No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18910.11968059016264787No Hit
CGGCCACCCTGACCATCAGCGGGGTCGAAGCCGGGGATGAGGGCGACTAT18690.11828821946800047No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18550.11740216538958849No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18140.11480729273138195No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACTTCCTATGTGGTGACTCAGC17830.1128453158434697No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17680.11189597218802827No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACTTCCTA17410.11018715360823372No Hit
GCACAGGCTCTGTGACTTCCTATGTGGTGACTCAGCCACCCTCGGTGTCA17360.10987070572308659No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17330.10968083699199829No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16790.10626319983240921No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16630.10525056659993835No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG16580.10493411871479122No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACTTC16560.10480753956073237No Hit
GTGTATTACTGTGCGAGGGGCCGAGCTGGACTAATTGATGCTTTTGATAT16540.10468096040667352No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16490.10436451252152637No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16330.10335187928905552No Hit
CCCCAGGGCAGACGGCCAGGATTACCTGTGGGGGAAGCAACATTGGAAGT16190.10246582521064353No Hit
GTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG16170.10233924605658469No Hit
CAGTAATAGTCGCCCTCATCCCCGGCTTCGACCCCGCTGATGGTCAGGGT16100.10189621901737869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)