FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074458
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27380.25482615420984345No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24570.22867343348925692No Hit
GACCTAGGACGGTCAACTTGGTCCCTTCGCCGAACACCCAAGTATTGGTACCTGTATATGAGCTGCAGTAATAAT23280.21666738020471718No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21630.20131080042216634No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21580.2008454495196648No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT21440.1995424669926605No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21210.19740185284115339No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21060.1960058001336488No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20910.19460974742614418No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTTCGCCGAACACCCAAGTATTGGTACCTGTATATGAGCTG20870.19423746670414294No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTG20580.191538431469634No Hit
CTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAGCAGTGACGTT19090.1776709745750881No Hit
GTATATGAGCTGCAGTAATAATCACCCTCGTCCTCAGCCTGGAGACCAGT18980.1766472025895847No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18640.17348281645257424No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18430.17152834266206776No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18250.1698530794130622No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTT18240.1697600092325619No Hit
GTCAATAAGCGGCCCTCAGGGGTTTCTACTCGCTTCTCTGCCTCCAAGTC17230.16035992100203078No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17110.1592430788360271No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16780.15617176287951695No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16320.15189053457650276No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16030.14919149934199383No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15680.14593404302448304No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15530.14453799031697842No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTGTAATTGTGTTTCCTGGTACCAACAACACCCAGGCAAAGC15290.142304305984971No Hit
CCCAAGTATTGGTACCTGTATATGAGCTGCAGTAATAATCACCCTCGTCC15080.14034983219446456No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15000.13960527075046208No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14830.13802307768195687No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14760.1373715864184547No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCTCCTGGACAGTCGATCAG14470.13467255118394578No Hit
ATACTTGGGTGTTCGGCGAAGGGACCAAGTTGACCGTCCTAGGTCAGCCC14430.13430027046194454No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14390.1339279897399433No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13770.1281576385489242No Hit
GTATTGGTACCTGTATATGAGCTGCAGTAATAATCACCCTCGTCCTCAGC13610.12666851566091927No Hit
CATATACAGGTACCAATACTTGGGTGTTCGGCGAAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCC13110.12201500663590387No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12780.11894369067939371No Hit
GACTCAGCCTGCCTCTGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCT12340.11484860273738015No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12180.11335947984937522No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12180.11335947984937522No Hit
GACCATCACTGGTCTCCAGGCTGAGGACGAGGGTGATTATTACTGCAGCT12140.112987199127374No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAG12060.11224263768337152No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11940.11112579551736783No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11930.11103272533686752No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11800.1098228129903635No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11470.10675149703385335No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11370.10582079522885027No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAAACACAATTACAA11350.10563465486784965No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11020.1025633389113395No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10990.10228412836983855No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10780.1003296545793321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)