FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568991
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTTAGTTTCAGGGAGA82980.5288749266248182No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55990.3568535447303394No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA54160.34518999790311095No Hit
GTTTTTATAGTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT50660.3228826679056795No Hit
CTATAAAAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT49520.31561685184937327No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47520.3028698061365553No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38610.24608171748595115No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT38400.24474327768610527No Hit
GTATATTACTGTGCGAGAGGGGATATCTATTACTATGATAATAGTGGTCC38160.24321363220056713No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37930.24174772194359304No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCC37040.23607528660138907No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36930.23537419908718407No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTT33690.2147239850324189No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAG33030.21051745994718896No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT32720.2085416678617022No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30470.19420124143478196No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30290.19305400732062836No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC29610.18872001177827025No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28620.18241022415042535No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28160.1794784036364772No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA28000.17845863997945177No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27440.17488946717986273No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27130.17291367509437594No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26620.16966317843760736No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCACTATAAAAACTGG26390.1681972681806333No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCACATGGGCCAGTTTTTATAGTG26040.16596653518089013No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26010.16577532949519788No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25950.16539291812381335No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25900.16507424198099288No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC25610.16322592035263428No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA25600.16316218512407019No Hit
GGCCAAAAGTGCACATGGGCCAGTTTTTATAGTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTG25230.16080398166719886No Hit
CCAGTGGCCCTGGTAGAAGCATCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAA23800.151689843982534No Hit
CATCTATGATGCTTCTACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23680.15092502123976492No Hit
TCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGC23270.14831187686863723No Hit
GTCTCCGGTGGCTCCATCAGTAGTAGTAGTTACTACTGGGGCTGGATCCG23220.1479932007258168No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23190.14780199504012453No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT22670.14448776315479184No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21280.13562856638438334No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20950.13352530384176836No Hit
GTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20710.13199565835623023No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20510.1307209537849484No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20120.1282352798709489No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19730.1257496059569494No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19300.12300899112869354No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT19290.12294525590012945No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19290.12294525590012945No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18990.12103319904320675No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18990.12103319904320675No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18910.12052331721469403No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18730.11937608310054042No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18670.11899367172915588No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18230.11618932167233591No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTCATGACGCAGTC18150.11567943984382319No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18120.11548823415813093No Hit
GTCAGAGTGTCAGTAGCAAGTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCCCCTCATCTATGATG18110.11542449892956684No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACT17840.1137036477583364No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17790.11338497161551596No Hit
CAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17790.11338497161551596No Hit
GGAGAAATAGTCATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG17590.11211026704423416No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17330.11045315110156784No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGC17280.11013447495874737No Hit
CACCTGCACTGTCTCCGGTGGCTCCATCAGTAGTAGTAGTTACTACTGGG16970.10815868287326058No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16850.10739386013049151No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16630.10599168510208154No Hit
GGTAGAAGCATCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCT16600.10580047941638926No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16600.10580047941638926No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16570.10560927373069698No Hit
GAGTGTCAGTAGCAAGTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCCCCTCATCTATGATGCTT16460.104908186216492No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16380.10439830438797928No Hit
CCCAGATACCACTGGAGAAATAGTCATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCAC16220.10337854073095384No Hit
GTTTATTACTGTCAGCACTATAAAAACTGGCCCATGTGCACTTTTGGCCA16210.10331480550238975No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTTCCAGCAGAAACCTGGCCAGG16180.10312359981669748No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16130.10280492367387703No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT16000.10197636570254387No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15770.10051045544556979No Hit
GGACACCAGTGGCCCTGGTAGAAGCATCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGG15740.10031924975987754No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTCATGACGCAGTCTCCAGCCACC15690.10000057361705707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)