FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568991
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTTAGTTTCAGGGAGA80920.5157454695406156No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55220.3519459321309045No Hit
GTTTTTATAGTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT53460.3407285319036247No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA49620.31625420413501415No Hit
CTATAAAAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT43650.27820427268225245No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40020.25506838471348786No Hit
GTATATTACTGTGCGAGAGGGGATATCTATTACTATGATAATAGTGGTCC39970.2547497085706674No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37940.24181145717215713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35920.228936941002211No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34810.221862330631597No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCC34120.2174645998606748No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT33470.21332181000400896No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33330.2124295168041117No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT32690.20835046217600994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32290.20580105303344634No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTT31320.19961873586272963No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC28020.17858611043657993No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAG28000.17845863997945177No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27140.17297741032294003No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA26500.16889835569483827No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCACATGGGCCAGTTTTTATAGTG26310.16768738635212058No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26210.16705003406647967No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25990.1656478590380697No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25210.16067651121007068No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCACTATAAAAACTGG25160.16035783506725024No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24620.15691613272478935No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24580.156661191810533No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24410.15557769292494347No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA23950.15264587241099534No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23600.1504151394112522No Hit
GGCCAAAAGTGCACATGGGCCAGTTTTTATAGTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTG23480.14965031666848314No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC23420.1492679052970986No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT23330.1486942882400218No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22380.14263944152643324No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21890.13951641532679282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21880.13945268009822873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21700.1383054459840751No Hit
CATCTATGATGCTTCTACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21490.13696700618422922No Hit
TCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGC20820.1326967458704352No Hit
GTCTCCGGTGGCTCCATCAGTAGTAGTAGTTACTACTGGGGCTGGATCCG20710.13199565835623023No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20620.1314220412991534No Hit
CCAGTGGCCCTGGTAGAAGCATCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAA20470.13046601287069207No Hit
GTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19240.122626579757309No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18760.11956728878623268No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18690.11912114218628404No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTCATGACGCAGTC18430.11746402624361772No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18010.11478714664392593No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17750.1131300307012596No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17690.11274761932987505No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17410.11096303293008054No Hit
GTCAGAGTGTCAGTAGCAAGTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCCCCTCATCTATGATG17340.11051688633013192No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17260.11000700450161921No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17160.1093696522159783No Hit
GGAGAAATAGTCATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG17050.1086685647017733No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16930.10790374195900423No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16680.10631036124490198No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16640.1060554203306456No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16580.10567300895926107No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGC16330.10407962824515882No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTCATGACGCAGTCTCCAGCCACC16160.1029961293595693No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16050.10229504184536431No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16030.10216757138823614No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15960.10172142478828751No Hit
CAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15810.10076539635982616No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15710.10012804407418525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT18250.075.64017570
CTACGGA18900.046.97168769
GGTATCA13550.040.0059971
GGGCGTA603.2178832E-739.739853
TCTACGG20300.038.72626568
GTATCAA33250.037.7331851
TTCTACG20250.036.7348167
ACGTTTC20250.035.79950363
GACGTTT20350.035.3476762
GGACGTT20750.034.5832861
TTGGACG20700.034.33650259
TTTCTAC21100.034.322866
TGGACGT21200.033.93665360
AGCCGTG21650.033.6575910
CCGTGTC21350.033.65313712
CGTGTCT21450.033.34613
CAGCCGT22000.033.1221289
ATCAACG37550.032.9288143
TATCAAC37700.032.9049342
GCCGTGT21850.032.88199611
Produced by FastQC (version 0.11.5)