FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904526
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26160.28921225039412907No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23200.25648792848408997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19660.2173514083619487No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18550.20507978764568402No Hit
GATCTATGGTGCATCTAATTTGGGAAGTGGGGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA18500.2045270119377442No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17810.1968987071681743No Hit
GTCCTGATCTATGGTGCATCTAATTTGGGAAGTGGGGTCCCATCACGGTT15510.1714710246029412No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15070.16660659837307054No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15030.16616437780671867No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAACAGAAA14520.16052606558573218No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14480.1600838450193803No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14370.15886773846191263No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTA13210.14604334203770816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13200.1459327868961202No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13000.14372168406436078No Hit
CCATAGATCAGGACCTTGGGGGCTTTCCCTGGTTTCTGTTGATACCAATT12840.14195280179895325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.1399628092503698No Hit
ACTTACTACTGTCAACAGAATTACAATACCCCTGCGATCACCTTCGGCCA12480.13797281670178635No Hit
GAATTACAATACCCCTGCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT12220.13509838302049915No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11930.13189228391444802No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG11930.13189228391444802No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11780.13023395679062846No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAATTACAATACC11100.12271620716264652No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11080.12249509687947058No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10920.12072621461406306No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10750.11884677720706757No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA10680.11807289121595178No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10410.11508790239307659No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10270.11354013041084501No Hit
GATCAGGACCTTGGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAAT10120.11188180328702546No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10000.11055514158796982No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9930.10978125559685403No Hit
GTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9850.10889681446415028No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9490.10491682936698338No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9470.10469571908380744No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT9450.1044746088006315No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9380.10370072280951571No Hit
GTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAATTACAAT9350.1033690573847518No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC9280.10259517139363601No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9090.10049462370346458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT200.00773253451.10510312
GTGGTAT3950.050.0381
ATCAACG49200.033.6620183
AACGCAG49650.033.631476
TCAACGC49800.033.1880264
CAACGCA50200.033.059355
CGCAGAG52550.031.7684738
ACGCAGA53050.031.4690537
AGAGTAC54650.030.67241111
GTATCAA55050.030.4562761
GCAGAGT55100.030.2982449
CACTCGG450.004914545530.28450410
CAGAGTA57950.028.86695910
TATCAAC58850.028.431782
TCGTATG1700.028.42061640
CGGGTCA2000.027.26660317
GAGTACG38000.027.25605212
AGTACGG38300.027.13301313
TCGCACG1250.001045356325.69101770
CGTATGC1901.8189894E-1225.44892541
Produced by FastQC (version 0.11.5)