FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904526
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25230.2789306222264479No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24150.2669906669349471No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19180.21204476156572613No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16200.17909932937251113No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15870.1754510097001081No Hit
GATCTATGGTGCATCTAATTTGGGAAGTGGGGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA15810.1747876788505803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15320.16937047691276977No Hit
GTCCTGATCTATGGTGCATCTAATTTGGGAAGTGGGGTCCCATCACGGTT14930.16505882639083896No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13640.15079721312599084No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13570.15002332713487507No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12730.1407366952414856No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12200.1348772727373232No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTA11930.13189228391444802No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAACAGAAA11870.1312289530649202No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11700.12934951565792469No Hit
ACTTACTACTGTCAACAGAATTACAATACCCCTGCGATCACCTTCGGCCA11450.12658563711822546No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG11340.12536953056075778No Hit
GAATTACAATACCCCTGCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT11200.1238217585785262No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11070.1223845417378826No Hit
CCATAGATCAGGACCTTGGGGGCTTTCCCTGGTTTCTGTTGATACCAATT11040.1220528763131187No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10960.12116843518041494No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10860.12006288376453524No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10840.1198417734813593No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10700.11829400149912772No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10640.11763067064959991No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAATTACAATACC10490.11597234352578037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10410.11508790239307659No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10130.11199235842861344No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9670.10690682191556683No Hit
GTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAATTACAAT9640.10657515649080292No Hit
GTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9360.10347961252633976No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9280.10259517139363601No Hit
GATCAGGACCTTGGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAAT9120.1008262891282285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4350.049.2232971
AACGCTA400.002168021635.79051661
ATCAACG44900.032.1547933
TCAACGC45150.032.105914
CAACGCA45750.031.6848495
AACGCAG46200.031.5966726
GTATCAA47600.030.988621
CTCGCTA1351.0913936E-1130.22283623
ACGCAGA48750.029.9439227
CGCAGAG49350.029.5110728
AATGGTA5500.029.34023370
TCGTTAA1154.91295E-429.23392770
AGAGTAC50400.028.96041311
TATCAAC51500.028.5051822
CGGCGAA500.006616846728.49619359
GCAGAGT51550.028.3174829
TCGCTAT1452.7284841E-1128.15409724
CAGAGTA53250.027.5394310
CGTAGTA500.00832663727.18552
CAGTCCG500.00836718527.158444
Produced by FastQC (version 0.11.5)