FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689786
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATAGGGAGAGCAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC95620.5658704711720893No Hit
CCCTATAGATGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG65260.3862027499340153No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57740.34170007326371504No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50740.300274709341893No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCACTGT50520.29897276933292144No Hit
GCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAATACCGC50220.2971973965934148No Hit
CCTATGAGCTGACTCAGCCACTCTCACTGTCAGTGGCCCTGGGACAGACG49750.294415979301521No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45870.27145449187056825No Hit
GACCTAGGACGATCAGCTTGGTCCCTCCGCCGAATACCGCAGCGCCGTCGTCCCACACCTGACAGTAATAGTCAG41410.2450606171432359No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT40580.24014875256393414No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39850.23582867889780124No Hit
GGATTACCTGTCGGGGAAACAACATTGGAAGTAAAAATGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCC38460.22760278520475374No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTC37860.22405203972574042No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36880.21825248877668535No Hit
GACTATTACTGTCAGGTGTGGGACGACGGCGCTGCGGTATTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAG36560.21635875785454486No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34810.20600241687408938No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32740.19375234497149343No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31660.18736100310926945No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGG31610.187065107652685No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG31170.1844612276347419No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31120.18416533217815748No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT30150.17842496032041927No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG29350.17369063301506818No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29340.1736314539237513No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT28340.16771354479206244No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28180.1667666793309922No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27830.1646954111349011No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27730.16410362022173222No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27660.16368936658251398No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27570.163156754760662No Hit
CTATTACTGTCAGGTGTGGGACGACGGCGCTGCGGTATTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCC27210.161026307473254No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACATTTTTACTT26380.15611444289395227No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26120.15457578651971315No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25970.15368810014995982No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25770.15250451832362205No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25220.14924966830119318No Hit
CCCCTGTGTTGGTCATCTATAGGGAGAGCAACCGGCCCTCTGGGATCCCT24900.14735593737905273No Hit
CTCTCACTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTCGGGGAAACAACATTGGAAGTAAAAATGTA24440.14463369917847585No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23800.140846237334195No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCA22690.13427735819802034No Hit
CGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACGACGGCG22030.1303715381711057No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21930.1297797472579368No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21670.1282410908836977No Hit
CAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGT21610.12788601633579635No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT21190.12540049450048704No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20990.12421691267414928No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20810.12315168903044528No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20580.12179056993015683No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG20380.12060698810381906No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19970.11818064535982663No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTCGGGGAAACAACATTGGAAGT19930.11794392899455908No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCA19530.11557676534188353No Hit
GTGTTGGTCATCTATAGGGAGAGCAACCGGCCCTCTGGGATCCCTGAGCG19360.11457072078949641No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19250.11391975078501064No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19040.112676989867356No Hit
GCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACGACGG18850.1115525871323351No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18770.1110791544018No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAATACCGCAG18660.11042818439731422No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGC18650.11036900530599733No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18550.10977721439282843No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18480.10936296075361022No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG17990.10646318527908268No Hit
GTATTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC17920.10604893163986447No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17540.10380012616982269No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17400.10297161889138624No Hit
GGCCAGGCCCCTGTGTTGGTCATCTATAGGGAGAGCAACCGGCCCTCTGG17340.10261654434348491No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17300.10237982797821736No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17240.10202475343031603No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTGTTCCC17120.10131460433451336No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAATACCGCAGCGCCGT17050.10090035069529515No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16900.10001266432554182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA309.260841E-657.35978338
GTCGTAT200.00741087251.65923739
GGTATCA13600.048.7241481
CGCGTAT507.044582E-848.2152939
ATCGCGT306.422559E-445.8386137
TCGTATG2400.044.5348540
CGTATGC2900.044.0517941
ACGAGAC4300.043.6841922
TATACAC4800.041.983573
GTATCAA38700.041.4828261
AGGGGGG4900.040.38010470
CCGTCTT3150.039.7293447
CCCACGA5000.039.6140719
CACGAGA4850.039.43099621
GTGGTAT2700.039.2174531
TGGTATC2800.039.0355722
CGAGACA4900.039.0344223
GTATGCC3450.038.06398842
CTTATAC5200.037.441481
TTATACA5200.037.440372
Produced by FastQC (version 0.11.5)