FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004686487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699424
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATACTGACTCAGTCACCCTCGGTGTCAGTGGCCCCAGGACAGACG81991.1722503088255478No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACTCCGAGATCACTACTAGCATCCCACAC25640.3665873633161001No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23340.33370316145857165No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21780.3113990941117262No Hit
GCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTATCAGCAGAAGTCAGGCCAGGCCC20620.29481401839227706No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACTCCGAGATCACTA20140.2879512284394016No Hit
CCCTGACCCTCAGCGGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATGCTAGTAGTGAT17730.25349430388433913No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17350.24806126183831265No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16640.23791005169968432No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16300.2330489088163975No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15460.22103902639886536No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15310.21889440453859177No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT14750.210887816260237No Hit
GGGTCAGGGTGGCCGTGTTCCCAGAGTTGGACCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCAT14230.20345312714462188No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14200.20302420277256714No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14200.20302420277256714No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGATACCAGTGCACACTTTTACTT14080.20130850528434827No Hit
CCCCAGGACAGACGGCCAGCATTACCTGTGGGGGAAACAACATTGGAAGT13790.19716223635448596No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13460.19244406826188407No Hit
CTACTAGCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC13160.18815482454133686No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13160.18815482454133686No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13100.18729697579722743No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12810.18315070686736515No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12490.17857551356544812No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12440.1778606396120236No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12420.1775746900306538No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12340.17643089170517456No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12320.17614494212380472No Hit
CTATTACTGTCAGGTGTGGGATGCTAGTAGTGATCTCGGAGTGTTTGGCG12130.1734284211007915No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12070.17257057235668208No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG11630.16627968156654618No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11380.16270531179942352No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA11100.15870201766024614No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACA10820.15469872352106875No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10570.15112435375394612No Hit
GGCTCTGTGACCTCCTATATACTGACTCAGTCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTAC10570.15112435375394612No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10400.1486937823123027No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACT10200.14583428649860458No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10170.14540536212654986No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT9830.14054421924326302No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9770.1396863704991536No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTG9750.13940042091778376No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACTCAGTCAC9570.13682687468545546No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGCTGAGGGTCAGGGTGG9390.13425332845312715No Hit
GCACAGGCTCTGTGACCTCCTATATACTGACTCAGTCACCCTCGGTGTCA9310.1331095301276479No Hit
GAGTTGGACCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGC9280.13268060575559318No Hit
CGGCCACCCTGACCCTCAGCGGGGTCGAAGCCGGGGATGAGGCCGACTAT9240.13210870659285356No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGGTCCAACTCTGGGAACACGG9230.13196573180216864No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9180.1312508578487441No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACTCCGAGATCA9160.1309649082673743No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACTCCGAGATCACTAC9110.13025003431394977No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9050.1293921855698403No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8840.12638971496545728No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG8830.1262467401747724No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGCTGAGGGTCAGGGT8770.12538889143066295No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8410.12024179896600633No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8360.11952692501258178No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA8200.11723932836162328No Hit
GGGTGGCCGTGTTCCCAGAGTTGGACCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA8150.11652445440819875No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG8040.11495173171066476No Hit
GTCCAACTCTGGGAACACGGCCACCCTGACCCTCAGCGGGGTCGAAGCCG8030.11480875691997987No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7950.11366495859450063No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7840.11209223589696664No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7810.11166331152491192No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7760.1109484375714874No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7720.11037653840874778No Hit
GTATATAGGAGGTCACAGAGCCTGTGCAGTGAGAGAGGAGGCCGAGGAGGAGAACGGTCCAGGCCATGGCTGAGG7710.11023356361806287No Hit
AAGTAAAAGTGTGCACTGGTATCAGCAGAAGTCAGGCCAGGCCCCTGTGC7690.10994761403669306No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7630.10908976529258362No Hit
CTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGA7570.10823191654847418No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG7560.10808894175778927No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7560.10808894175778927No Hit
GCTTGGTCCCTCCGCCAAACACTCCGAGATCACTACTAGCATCCCACACCTGACAGTAATAGTCGGCCTCATCCC7380.10551539552546094No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7280.10408564761861189No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7140.10208400054902321No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC7070.10108317701422885No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7040.10065425264217413No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG7010.10022532827011942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)