FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699424
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATACTGACTCAGTCACCCTCGGTGTCAGTGGCCCCAGGACAGACG70361.0059706272590017No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22000.3145445395067942No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACTCCGAGATCACTACTAGCATCCCACAC21970.3141156151347394No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21860.31254289243720546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17500.25020588369858626No Hit
GCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTATCAGCAGAAGTCAGGCCAGGCCC17460.24963398453584665No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACTCCGAGATCACTA16860.24105549709475227No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15950.2280447911424258No Hit
CCCTGACCCTCAGCGGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATGCTAGTAGTGAT15220.2176076314224276No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15080.21560598435283893No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14460.20674154733037473No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14360.20531179942352562No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14360.20531179942352562No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT13950.19944983300544447No Hit
GGGTCAGGGTGGCCGTGTTCCCAGAGTTGGACCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCAT13920.19902090863338975No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACA13350.1908713455643501No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13310.19029944640161048No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGATACCAGTGCACACTTTTACTT13210.1888696984947614No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13000.18586722789037838No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12590.1800052614722972No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12560.17957633710024248No Hit
CCCCAGGACAGACGGCCAGCATTACCTGTGGGGGAAACAACATTGGAAGT12270.1754300681703802No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11880.16985405133366885No Hit
CTATTACTGTCAGGTGTGGGATGCTAGTAGTGATCTCGGAGTGTTTGGCG11660.1667086059386009No Hit
CTACTAGCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC11280.16127556389257447No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11160.15955986640435557No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10920.15612847142791783No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10870.1554135974744933No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10850.15512764789312347No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10850.15512764789312347No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10470.14969460584709704No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG10190.14569131170791966No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT10130.14483346296381022No Hit
GGCTCTGTGACCTCCTATATACTGACTCAGTCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTAC9980.1426888411035366No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9950.1422599167314819No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACT9920.14183099235942717No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA9830.14054421924326302No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9760.1395433957084687No Hit
GGGTGGCCGTGTTCCCAGAGTTGGACCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA9520.13611200073203092No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9430.13482522761586677No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9310.1331095301276479No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9240.13210870659285356No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9190.13139383263942903No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8820.12610376538408746No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG8740.12495996705860822No Hit
GCACAGGCTCTGTGACCTCCTATATACTGACTCAGTCACCCTCGGTGTCA8620.12324426957038934No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACTCCGAGATCA8550.122243446035595No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACTCAGTCAC8540.1221004712449101No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8520.12181452166354029No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8480.12124262250080066No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTG8330.11909800064052706No Hit
GAGTTGGACCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGC8290.11852610147778744No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8200.11723932836162328No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG8120.11609553003614403No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA8000.11437983254792515No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGGTCCAACTCTGGGAACACGG7940.11352198380381572No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACTCCGAGATCACTAC7930.1133790090131308No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7920.11323603422244591No Hit
CGGCCACCCTGACCCTCAGCGGGGTCGAAGCCGGGGATGAGGCCGACTAT7850.11223521068765155No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7780.1112343871528572No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGCTGAGGGTCAGGGTGG7760.1109484375714874No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7720.11037653840874778No Hit
GTCCAACTCTGGGAACACGGCCACCCTGACCCTCAGCGGGGTCGAAGCCG7410.10594431989751567No Hit
CTCCGAGATCACTACTAGCATCCCACACCTGACAGTAATAGTCGGCCTCA7390.10565837031614586No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7230.10337077366518736No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC7190.10279887450244773No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7160.10236995013039302No Hit
CTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGA7100.10151210138628357No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC7050.10079722743285904No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGCTGAGGGTCAGGGT7050.10079722743285904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)