FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935317
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41230.4408131146980115No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40470.43268752733030613No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30340.32438200096865555No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29850.3191431354289508No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29740.31796706357309873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29270.3129420292799126No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28510.3048164419122073No Hit
GTTTATTACTGTCAGTGCCGTTACGCCGGGCCTCCGGGATTTACATTCGGCCCTGGGACCAAAGTGGAAATCAAA27540.29444562645605715No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27080.28952750778613023No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGACCTAGAGCCTGAAGATT24960.26686139565516287No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGGTCGCTGATGGTGAGAGTGA24580.2627986019713103No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23980.25638366457575346No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGGGGCCCTATT23410.25028947404997454No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23240.24847190845456676No Hit
GTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGCGC22730.24301921166834345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22400.2394909961007872No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22390.23938408047752793No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAATGTAAATCCCGGAGG21140.22601962757011793No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGCGCCACCCTCTCCT20940.22388131510493234No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20550.2197116057978204No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAATGTA20520.21939085892804258No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20220.21618339023026417No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAATAGGGCCCCTGGCATCCCA19650.21008919970448522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19560.20912695909515172No Hit
GACTTCACTCTCACCATCAGCGACCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGTGCCGTTACGCCGGG19100.2042088404252248No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18930.20239127482981706No Hit
GTCTTTGTCTCCAGGGGAGAGCGCCACCCTCTCCTGCAGGGCCAGTCGGAGTCTTCTCAAATATTTAGCCTGGTA18870.20174978109026137No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCA18500.19779390302966804No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17750.18977523128522203No Hit
GTCTGGGAGCCTGGCCAGGCTTTTGTTGGTACCAGGCTAAATATTTGAGA17470.18678159383396217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17400.18603318447114722No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16400.1753416221452192No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16250.17373788779633No Hit
CTCCTGCAGGGCCAGTCGGAGTCTTCTCAAATATTTAGCCTGGTACCAACAAAAGCCTGGCCAGGCTCCCAGACT16000.171064997214848No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15980.17085116596832944No Hit
CTATTGGATGCATCATAGATGAGGAGTCTGGGAGCCTGGCCAGGCTTTTG15930.17031658785203305No Hit
ATCCAATAGGGCCCCTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG15810.1690336003729217No Hit
TCTATGATGCATCCAATAGGGCCCCTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACT15560.1663607097914397No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15040.16080109738195714No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14900.15930427865632724No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14770.15791437555395657No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14710.15727288181440088No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13910.1487196319536585No Hit
CTCCAGGGGAGAGCGCCACCCTCTCCTGCAGGGCCAGTCGGAGTCTTCTC13670.14615365699543575No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13620.14561907887913939No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13610.1455121632558801No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13580.14519141638610225No Hit
CAATAGGGCCCCTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGA13400.14326693516743522No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13210.1412355383255089No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13140.1404871289626939No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT13080.13984563522313825No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13000.138990310237064No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12890.13781423838121193No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12610.1348206009299521No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12580.13449985406017426No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12540.13407219156713712No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12480.13343069782758143No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12310.1316131322321737No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12100.1293679041437288No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11760.1257327729529133No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11640.12444978547380194No Hit
CAGTAATAAACTGCAGAATCTTCAGGCTCTAGGTCGCTGATGGTGAGAGT11630.12434286985054264No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11510.1230598823714313No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11440.12231147300861633No Hit
ACCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGTGCCGTTACGCCGGGCCTCCGGGATTTACATTCGGCC11170.11942475118061577No Hit
CACCATCAGCGACCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGT11130.11899708868757863No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11120.11889017306431937No Hit
GCGTAACGGCACTGACAGTAATAAACTGCAGAATCTTCAGGCTCTAGGTCGCTGATGGTGAGAGTGAAGTCTGTC11080.11846251057128225No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10990.11750026996194872No Hit
ATGTAAATCCCGGAGGCCCGGCGTAACGGCACTGACAGTAATAAACTGCA10750.11493429500372601No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10640.11375822314787393No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10610.11343747627809608No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10590.11322364503157753No Hit
ACAAAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCAATAGGGCCCCTGGCATCCCAGCCAGGTT10580.11311672940831825No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10490.11215448879898474No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10410.1112991638129105No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10320.11033692320357696No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10240.10948159821750274No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10140.10841244198490994No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10030.10723637012905786No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGACAAACTGTCTTGACACAGTC9970.10659487638950216No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9960.1064879607662429No Hit
CCCAGATACCACCGGACAAACTGTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGCGCCAC9830.10509805766387224No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9730.10402890143127945No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9710.1038150701847609No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9680.10349432331498305No Hit
CATCATAGATGAGGAGTCTGGGAGCCTGGCCAGGCTTTTGTTGGTACCAGGCTAAATATTTGAGAAGACTCCGA9590.10253208270564952No Hit
TTATTACTGTCAGTGCCGTTACGCCGGGCCTCCGGGATTTACATTCGGCC9530.10189058896609385No Hit
GCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAATGTAAATCCCGGAGGCCCGGCGTAACGGCACTGA9430.10082143273350105No Hit
CCGTTACGCCGGGCCTCCGGGATTTACATTCGGCCCTGGGACCAAAGTGG9410.10060760148698249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTG200.00698250152.4410747
TATCGTC200.007197147752.0410943
TCGTATG2450.045.0256240
GGTATCA8850.044.32191
ATCTCGT2550.042.98802637
ATCTCCG3100.041.4323710
TCTCCGA3100.041.4323711
GACGGAC2950.041.33595726
CGTATGC2600.041.21446641
ATGCCGT2400.040.5453744
TATCTCG2700.040.5448836
GACCGGA700.001237732540.1125870
CGAGACG3050.039.9314823
TCCTATC2750.039.6532333
GTATCAA25050.039.551551
CGTATGG350.001334887139.5051141
CTCCTAT2950.039.23499732
CTTATAC3400.038.787281
ACGAGAC3150.038.65967622
CGAGCCC3200.038.025115
Produced by FastQC (version 0.11.5)