FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935317
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40280.4306561304883799No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35260.3769844876122213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33110.3539976286114761No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30470.3257719040710262No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28740.30727550124717073No Hit
GTTTATTACTGTCAGTGCCGTTACGCCGGGCCTCCGGGATTTACATTCGGCCCTGGGACCAAAGTGGAAATCAAA26920.28781685781398175No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26900.2876030265674632No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26120.2792636079532394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25840.27626997050197954No Hit
GTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGCGC24370.26055337388286537No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGGTCGCTGATGGTGAGAGTGA23880.25531450834316066No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGACCTAGAGCCTGAAGATT23640.25274853338493797No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22960.2454782710033069No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22430.23981174297056507No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGGGGCCCTATT21270.2274095306724886No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21210.2267680369329329No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20320.21725254646285697No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAATGTA19890.21265517466270795No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19570.20923387471841098No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19300.2063471528904104No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19200.20527799665781762No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGCGCCACCCTCTCCT18950.2026051060763356No Hit
GTCTTTGTCTCCAGGGGAGAGCGCCACCCTCTCCTGCAGGGCCAGTCGGAGTCTTCTCAAATATTTAGCCTGGTA18790.20089445610418713No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAATGTAAATCCCGGAGG18770.20068062485766855No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18740.20035987798789073No Hit
GACTTCACTCTCACCATCAGCGACCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGTGCCGTTACGCCGGG18560.1984353967692237No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18430.19704549366685303No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18280.19544175931796387No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18140.19394494059233394No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCA18020.19266195311322257No Hit
CTCCTGCAGGGCCAGTCGGAGTCTTCTCAAATATTTAGCCTGGTACCAACAAAAGCCTGGCCAGGCTCCCAGACT17820.19052364064803698No Hit
GTCTGGGAGCCTGGCCAGGCTTTTGTTGGTACCAGGCTAAATATTTGAGA17090.18271880015010952No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16790.17951133145233114No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16740.17897675333603472No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAATAGGGCCCCTGGCATCCCA16720.17876292208951616No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15860.1695681784892181No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15450.1651846379355876No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14940.15973194114936431No Hit
ATCCAATAGGGCCCCTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG14660.1567383036981045No Hit
TCTATGATGCATCCAATAGGGCCCCTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACT14050.15021645067928843No Hit
CTATTGGATGCATCATAGATGAGGAGTCTGGGAGCCTGGCCAGGCTTTTG13660.1460467413721765No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13580.14519141638610225No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13170.14080787583247178No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13070.13973871959987896No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12640.13514134779972994No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12580.13449985406017426No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT12510.13375144469735928No Hit
CTCCAGGGGAGAGCGCCACCCTCTCCTGCAGGGCCAGTCGGAGTCTTCTC12410.13268228846476648No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12200.1304370603763216No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12140.12979556663676595No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11840.1265880979389875No Hit
CAATAGGGCCCCTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGA11790.12605351982269114No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11640.12444978547380194No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11630.12434286985054264No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11350.1213492323992828No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11250.12028007616669001No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10980.11739335433868946No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGACAAACTGTCTTGACACAGTC10950.11707260746891161No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10940.11696569184565234No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10840.11589653561305954No Hit
CAGTAATAAACTGCAGAATCTTCAGGCTCTAGGTCGCTGATGGTGAGAGT10660.1139720543943925No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10630.11365130752461466No Hit
ATGTAAATCCCGGAGGCCCGGCGTAACGGCACTGACAGTAATAAACTGCA10540.11268906691528112No Hit
CACCATCAGCGACCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGT10380.11097841694313265No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10250.109588513840762No Hit
ACCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGTGCCGTTACGCCGGGCCTCCGGGATTTACATTCGGCC10200.10905393572446562No Hit
ACAAAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCAATAGGGCCCCTGGCATCCCAGCCAGGTT10130.10830552636165065No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9880.10563263578016865No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9820.10499114204061298No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9750.10424273267779802No Hit
GCGTAACGGCACTGACAGTAATAAACTGCAGAATCTTCAGGCTCTAGGTCGCTGATGGTGAGAGTGAAGTCTGTC9730.10402890143127945No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9650.1031735764452052No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9600.10263899832890881No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9570.10231825145913098No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9560.10221133583587169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)