FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444537
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG113450.4640960639990313No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC101700.416029702148096No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG87110.3563455983689345No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAATGCCAGTG83220.3404325645306248No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT75830.31020189099203654No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG74350.3041475747759187No Hit
CATCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG73380.3001795432018415No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC68850.2816484266754809No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGAT67920.2778440252694068No Hit
GTAATAGTCAGACTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC67450.27592137079536944No Hit
GCTTAACAGTGATGGCAGCCACATCAAGGGGGACGGGATCCCTGATCGCT66710.2728942126873105No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG66220.2708897431292715No Hit
CTGTTAAGCTTCATCAAGAACCGGGGGCCCTTCTCTGGCTGCCGCTGATGCCATGCGATGGAGTAGCTGCTGTGC65630.26847619815122453No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC60870.2490042081588456No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACTCCATCGCATGGCATCAG60620.24798151960882575No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT59970.24532252937877397No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC59800.24462710116476047No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA59310.24262263160672146No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT58110.2377137265666259No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG57980.23718192852061554No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG57850.23665013047460523No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC56030.22920495783046035No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT55800.228264084364442No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA55710.22789591648643484No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG54870.22445968295836796No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC53250.217832661154239No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACTCCATCGCATGGCATC53100.2172190480242271No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC53020.21689178768822073No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC52930.21652361981021354No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGTCTGACTATTACTGTCAGAC50650.20719670023403203No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG50430.20629673431001452No Hit
GTCTGAGGATGAGTCTGACTATTACTGTCAGACCTGGGGCACTGGCATTCGGGTGTTCGGCGGAGGGACCAAG49110.20089693876590947No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA49030.20056967842990311No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG47570.19459717729778686No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT46820.19152911164772715No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT46710.1910791286857184No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGA46170.1888701214176754No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC45620.18662020660763162No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA45260.18514753509560297No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC45230.18502481246960056No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT42950.17569789289341908No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA42750.17487974205340315No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC42720.17475701942740077No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC42210.17267073478536016No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41570.17005265209730924No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGTCTGACTATTACT41150.1683345353332758No Hit
CCCTTGATGTGGCTGCCATCACTGTTAAGCTTCATCAAGAACCGGGGGCC40650.166289158233236No Hit
CCCGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGACTCATCCTCA40630.1662073431492344No Hit
GTTCTTGATGAAGCTTAACAGTGATGGCAGCCACATCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG40360.1651028395152129No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT40330.16498011688921052No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT38310.1567167934050497No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38050.155653197313029No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC37940.15520321435102025No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG36860.1507851998149343No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC36600.14972160372291357No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA36110.1477171341648746No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC35010.143217304544787No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTG34930.14289004420878063No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA34930.14289004420878063No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC34880.14268550649877665No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG34440.14088557465074164No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC33470.13691754307666443No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC33410.13667209782465964No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT33250.1360175771526469No Hit
GCTTGGTCCCTCCGCCGAACACCCGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGACTCATCCTCAGA32990.1349539810606262No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA32180.13164047015856173No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT32050.1311086721125514No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC31780.13000416847852989No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC31750.1298814458525275No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC31530.12898147992850997No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC31410.12849058942450042No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG30810.12603613690445267No Hit
CTCTGAGCAGTGGGCACAGCAGCTACTCCATCGCATGGCATCAGCGGCAG30740.1257497841104471No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA30670.1254634313164415No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGC30670.1254634313164415No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC29690.1214544922003635No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC29660.12133176957436112No Hit
CTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCC29480.1205954338183468No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGATGTGGCT29130.11916366984831893No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC29020.11871368688631018No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC28840.11797735113029584No Hit
GAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGTCTGACTA28700.1174046455422847No Hit
CAGTAATAGTCAGACTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC28650.11720010783228071No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG28610.11703647766427754No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG28470.11646377207626638No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT28120.11503200810623851No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT27900.114132042182221No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG27270.11155486703617085No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA27180.11118669915816369No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC27150.1110639765321613No Hit
GAGTAGCTGCTGTGCCCACTGCTCAGAGTGCAGGTGAGCTTGACCGAGGC26950.11024582569214539No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG26700.10922313714212548No Hit
GTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGCGA26190.10713685250008488No Hit
GTCTGACTATTACTGTCAGACCTGGGGCACTGGCATTCGGGTGTTCGGCG26080.10668686953807613No Hit
ACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGTCTGACTATTACTGT25900.10595053378206179No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA25820.10562327344605543No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG25760.10537782819405066No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA25600.10472330752203793No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA25580.10464149243803633No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT25440.10406878685002517No Hit
GCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAG25120.1027597455059997No Hit
GCATCAGCGGCAGCCAGAGAAGGGCCCCCGGTTCTTGATGAAGCTTAACAGTGATGGCAGCCACATCAAGGGGGA24790.10140979661997344No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG24660.10087799857396308No Hit
GACCTGGGGCACTGGCATTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC24640.10079618348996149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)