FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224807
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT80990.6612470372883238No Hit
GTTTTATACAGCCCCAACAATAAGAACTACTTAGGTTGGTACCAACAGAA71600.5845818973928137No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56740.46325666002888616No Hit
GCTTAGGAGGGTGTCCTGGTTTCTGTTGGTACCAACCTAAGTAGTTCTTATTGTTGGGGCTGTATAAAACACT53260.4348440203232019No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45790.3738548195756556No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA41760.3409516764682109No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40040.3269086476481601No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35360.2886985459749985No Hit
GTAGTACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAGGGAGA34790.2840447515404468No Hit
GTTTATTACTGTCAGGAGTATTTTACTACTCGTCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA32390.2644498276054921No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31550.257591604228258No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29940.24444667608855924No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29160.238078325809699No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29030.2370169340965556No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27810.227056181096287No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27100.22125934943219625No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27070.22101441288300933No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26950.2200346666862616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26520.2165239094812489No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26390.21546251776810552No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25320.20672644751377156No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG24600.20084797033328516No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC24120.1969289855462942No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA23960.1956226572839639No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA23780.19415303798884231No Hit
GTATTTTACTACTCGTCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23750.1939081014396554No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22740.1856619042836953No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGGAGTATTTTACTACT22280.18190621052949568No Hit
ACTTAGGTTGGTACCAACAGAAACCAGGACACCCTCCTAAGCTGCTCATT21860.1784770988408786No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCCCCAA21450.1751296326686572No Hit
GTAAAATACTCCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC21400.17472140508667897No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT20730.1692511554881708No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19840.16198470452895844No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19660.16051508523383684No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC19580.1598619211026717No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19020.15528977218451562No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18980.15496319011893303No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18750.1530853432418332No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18720.15284040669264629No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC18560.15153407843031597No Hit
GTAAATGAGCAGCTTAGGAGGGTGTCCTGGTTTCTGTTGGTACCAACCTA18450.15063597774996387No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18390.15014610465159No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18290.14932964948763355No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18190.14851319432367713No Hit
GTGTACTACTGTGCGAAAAATCGGGGGGACAGTGGCTACCCGAGGTTTTG18060.14745180261053376No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGGTGTCCTGGTTTCTGTTGGTA17810.14541066470064262No Hit
GTATCAACGCAGAGTACGGGGAGCCACAACAGGCAGGCAGGGGCAGCAAG17640.14402269092191666No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17350.141654970946443No Hit
CTACTTAGGTTGGTACCAACAGAAACCAGGACACCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC17170.1401853516513214No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17150.1400220606185301No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGGAGTATTTTACTACTCGTCTCAGTTT17000.13879737787259544No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16860.13765434064305643No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT16520.1348783930856045No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16410.13398029240525242No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16280.13291890069210904No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16040.13095940829861358No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16030.13087776278221794No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCCCCAACAATAAGAACTA15500.12655055041324878No Hit
CAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCAT15410.12581574076568797No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15260.1245910580197533No Hit
GTACCAACAGAAACCAGGACACCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA15190.12401953940498381No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15180.12393789388858815No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15160.12377460285579688No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGGGCTGGGTCCG14880.12148852839671882No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14820.12099865529834496No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14610.11928409945403642No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGGAGTATTTTACTACTCGTC14590.11912080842124514No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14220.11609992431460629No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14210.11601827879821065No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14030.11454865950308905No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13930.11373220433913261No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13920.11365055882273697No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13560.11071132023249376No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13500.11022144713411991No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13390.10932334645376782No Hit
CAATAAGAACTACTTAGGTTGGTACCAACAGAAACCAGGACACCCTCCTAAGCTGCTCATTTACTGGGCATCTAC13370.10916005542097654No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG13280.10842524577341574No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13270.10834360025702008No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13270.10834360025702008No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13220.10793537267504187No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13010.10622081683073334No Hit
ATTTTACTACTCGTCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAA12980.10597588028154639No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12950.10573094373235946No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12950.10573094373235946No Hit
CCTAAGTAGTTCTTATTGTTGGGGCTGTATAAAACACTCTGGCTGGACTT12950.10573094373235946No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12950.10573094373235946No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12810.10458790650282043No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12740.10401638788805094No Hit
GCCCCAACAATAAGAACTACTTAGGTTGGTACCAACAGAAACCAGGACACCCTCCTAAGCTGCTCATTTACTGGG12660.10336322375688578No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12620.10303664169130321No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12350.10083221274862081No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12320.10058727619943386No Hit
CCTTGGTCCCTCCGCCGAAACTGAGACGAGTAGTAAAATACTCCTGACAGTAATAAACTGCCACATCTTCAGC12310.10050563068303824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT8900.057.05647770
TCGTAAT200.00784685850.9167942
GATCGTC200.00785319350.90647
GTGGTAT3200.044.5521931
GGTATCA11500.044.275471
ATCGTAT551.491735E-743.82666839
TGGTATC3350.042.5573232
CTCTTAT1550.041.6094251
GCTCTAA751.022272E-940.7317774
TGCTTGA4000.039.77597455
TCGTATG4200.039.41005740
CGTATGC3950.039.3555441
CGCTAAT4200.039.0729432
ACGAGAC4550.038.81752422
TCGCTAA4400.038.8094431
ATCTCGT3100.038.77249537
GTATCAA30850.038.5108151
GTCGTAT451.0127382E-438.26137539
CTTGAAA4300.038.0106757
CACGAGA4650.037.97653221
Produced by FastQC (version 0.11.5)